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  • Martin Philip Jordan, Christiane Köhne, F. M. Wurm
  • Biology, Medicine
  • Cytotechnology
  • 1998
  • This is the first report of transient transfection of suspended cells with purified plasmid DNA in bioreactors or spinner flasks. DNA/calcium phosphate complexes were pumped or injected directly intoExpand
  • Anita Kuhn, Dorothee Kiefer, Christiane Köhne, Hong Yin Zhu, William R. Tschantz, Ross E. Dalbey
  • Medicine, Biology
  • European journal of biochemistry
  • 1994 (First Publication: 1 December 1994)
  • We have studied the insertion of pro-OmpA into the Escherichia coli membrane in vivo using various mutants that have either alterations in the amino-terminal parts of the signal peptide or in theExpand
  • F M Wurm, A. H. Johnson, +6 authors William R Arathoon
  • Biology, Medicine
  • Annals of the New York Academy of Sciences
  • 1996 (First Publication: 1 May 1996)
  • Keywords: Animals ; CHO Cells ; Cricetinae ; Immunoglobulin G/biosynthesis ; Methotrexate/pharmacology ; Receptors ; Tumor Necrosis Factor/biosynthesis/genetics ; Recombinant FusionExpand
  • Gerhard Müller-Newen, Christiane Köhne, +6 authors Peter C. Heinrich
  • Medicine, Biology
  • European journal of biochemistry
  • 1996 (First Publication: 15 March 1996)
  • The soluble human interleukin-6 receptor (shIL6R) was purified from human plasma. In a single immunoaffinity purification step a 140000-fold enrichment with a yield of 95% was achieved. A subsequentExpand
  • Christiane Köhne, Andrea B. Johnson, +8 authors Phillip W Berman
  • Biology, Medicine
  • Journal of cellular biochemistry
  • 1999 (First Publication: 1 December 1999)
  • Strategies that prevent the attachment of N-linked carbohydrates to nascent glycoproteins often impair intracellular transport and secretion. In the present study, we describe a method to rescue theExpand