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In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations.
TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) is a method of choice for rapid identification and quantification of the apoptotic cell fraction in cultured cellExpand
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TUNEL Apoptotic Cell Detection in Tissue Sections: Critical Evaluation and Improvement
TUNEL, i.e., terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, has become a widely used staining method to assist in detection of apoptotic cells in tissue sections. However,Expand
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F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence.
We propose a general solution to the problem of using antibodies originating in the same species for double immunohistochemical labeling. It relies on the use of a two-step protocol in which aExpand
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Importance of DNA fragmentation in apoptosis with regard to TUNEL specificity.
In the absence of a universal specific molecular tracer of apoptosis, structural DNA alterations provide the basis of labeling systems: double-strand fragmentation for TUNEL (terminalExpand
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Fine characterization of intrahepatic NK cells expressing natural killer receptors in chronic hepatitis B and C.
BACKGROUND/AIMS The fate of intrahepatic NK cell subsets in the course of HCV and HBV infections is not clearly understood. METHODS Blood and intrahepatic CD56(+) NK cell subsets (expressing NKG2A,Expand
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Intermittent hypoxia induces early functional cardiovascular remodeling in mice.
RATIONALE Intermittent hypoxia, a hallmark of sleep apnea, is a major factor for hypertension and impaired vasoreactivity. OBJECTIVES To examine the temporal occurrence of these two outcomes inExpand
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The inflammatory preatherosclerotic remodeling induced by intermittent hypoxia is attenuated by RANTES/CCL5 inhibition.
RATIONALE The highly prevalent obstructive sleep apnea syndrome (OSA) with its main component intermittent hypoxia (IH) is a risk factor for cardiovascular mortality. The poor knowledge of itsExpand
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TUNEL: Improvement and Evaluation of the Method for In Situ Apoptotic Cell Identification
Introduction TUNEL, or terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, is acknowledged as a method of choice in the rapid identification and quantification of the apoptoticExpand
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Characterization and role of intra-hepatic regulatory T cells in chronic hepatitis C pathogenesis.
BACKGROUND & AIMS In chronic hepatitis C (CHC), HCV-specific T-cell responses are often dysfunctionnal. In vitro data point out that regulatory T cells (Treg) are able to suppress HCV-specificExpand
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PS-100 and NF 70-200 Double Immunolabeling for Human Digital Skin Meissner Corpuscle 3D Imaging
For detailed study of complex structures such as corpuscular mechanoreceptors, confocal microscopy can be used with multiple immunolabeling that identifies specifically different subcomponents. InExpand
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