Christian Ruppert

Learn More
During post-natal cerebellar development the steady-state levels of c-myc transcripts exhibit characteristic changes. As determined by the S1 nuclease protection assay the level of c-myc transcript, which is very high in the late embryonic cerebellum, decreased to low levels shortly after birth. One week later there is a second period of c-myc mRNA(More)
We have used a retroviral cDNA expression system to drive the expression of the different forms of glutamic acid decarboxylase (GAD65, GAD67, or both). Individual clones of engineered Rat-1 cells make the appropriate GAD mRNAs and GAD polypeptides, show GAD enzymatic activity, and make GABA. Clones expressing GAD65 had higher enzymatic activity than those(More)
Cloned cDNA from adult mouse cerebellum composed of subsequences homologous to the L1Md consensus sequence (long interspersed repetitive element "LINE" family of the mouse) hybridizes specifically with large nuclear poly(A)+RNAs that are highly concentrated in the murine and rat cerebellum. These homogeneous L1-related transcripts were localized in the(More)
The proto-oncogene c-fos can be induced in cells to high transcript levels by numerous exogenous stimuli. We show here by S1 nuclease protection assay that mere mechanical disaggregation and incubation at 37 degrees C induces high levels of c-fos hnRNA and subsequently of mature mRNA in neonatal mouse cerebellar tissue. This specific increase of c-fos(More)
Genotype- and age-specific proteins are found in the developing cerebellum of wildtype and staggerer (sg/sg) mutant mice by analysis of silver-stained one- and two-dimensional protein patterns. The methods used are suitable for gross inspection of protein patterns without previous purification, labeling, or immunochemistry. SP47, a polypeptide of 47 kD with(More)
  • 1