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At the end of cytokinesis, the dividing cells are connected by an intercellular bridge, containing the midbody along with a single, densely ubiquitylated, circular structure called the midbody ring (MR). Recent studies revealed that the MR serves as a target site for membrane delivery and as a physical barrier between the prospective daughter cells. The MR(More)
Cortical flows mediate anteroposterior polarization in Caenorhabditis elegans by generating two mutually exclusive membrane domains. However, factors downstream of anteroposterior polarity that establish the dorsoventral axis remain elusive. Here, we show that rotational cortical flow orthogonal to the anteroposterior axis during the division of the AB(More)
22 Over the past few decades, many researchers have concluded that adequately addressing issues in the knowledge society requires a major transformation of research, away from disengaged disciplines toward socially engaged, transdisciplinary approaches. To date, knowledge exchange between transdisciplinarity researchers via academic publications has been(More)
Autophagy can act either as a tumor suppressor or as a survival mechanism for established tumors. To understand how autophagy plays this dual role in cancer, in vivo models are required. By using a highly heterogeneous C. elegans germline tumor, we show that autophagy-related proteins are expressed in a specific subset of tumor cells, neurons. Inhibition of(More)
Asymmetric cell divisions combine cell division with fate specification and one general model of how this is achieved was proposed already decades ago(1,2): During interphase, the cell polarity axis is specified, followed by orientation of the spindle along the polarity axis and segregation of fate determinants along the polarity axis during mitosis. In(More)
The midbody is a protein-dense assembly that forms during cytokinesis when the actomyosin ring constricts around bundling central spindle microtubules. After its initial description by Walther Flemming in the late nineteenth century and its rediscovery through electron microscopy in the 1960s and 1970s, its ultrastructural organization and the sequential(More)
Quantitatively capturing developmental processes is crucial to derive mechanistic models and key to identify and describe mutant phenotypes. Here protocols are presented for preparing embryos and adult C. elegans animals for short- and long-term time-lapse microscopy and methods for tracking and quantification of developmental processes. The methods(More)
Teile dieser Arbeit (Text und Abbildungen) sind daher diesen Veröffentlichungen entlehnt. Contents i Contents 1 Summary 1 2 Introduction 2 2.1 Cytokinesis as the final process in cell division 2 2.2 The timing of cytokinesis 3 2.3 Cleavage site specification 4 2.4 The spindle midzone and the formation of the midbody 6 2.5 Links between mitotic exit and(More)
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