Christian Jurinke

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Here we devise a new method for high-throughput comparative sequence analysis. The developed protocol comprises a homogeneous in vitro transcription/RNase cleavage system with the accuracy and data acquisition speed of matrix-assisted laser desorption/ionization coupled with time-of-flight mass spectrometry (MALDI-TOF MS). In summary, the target region is(More)
A rapid approach to the 16S rRNA gene (16S rDNA)-based bacterial identification has been developed that combines uracil-DNA-glycosylase (UDG)-mediated base-specific fragmentation of PCR products with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). 16S rDNA signature sequences were PCR-amplified from both cultured(More)
Capture Compound mass spectrometry (CCMS) is an innovative technology to reduce biological sample complexity by selective isolation of targeted protein or enzyme families. Small synthetic molecules (Capture Compounds™) are used to interrogate native proteins. Using staurosporine as a selectivity function for a kinase-specific Capture Compound™ enables(More)
application notes proteomics cal sample to allow a reversible, affinity-driven interaction with the targets. In the second step, the reactivity function is activated using UV light and covalently attached to the proteins using caprotec's caproBox™. This instrument combines timed UV irradiation and temperature control in one system and allows a convenient(More)
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