Christa Kirsten

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Cercarial secretions from different species of the parasite Schistosoma and from Trichobilharzia ocellata contain a proteolytic activity, cercarial elastase, which was demonstrated by a 30 kDa band in gelatin gels. Sera of patients infected with Schistosoma mansoni, Schistosoma haematobium or Schistosoma japonicum contain immunoglobulin G which react in(More)
A purified 41-kDa protein of the rodent filaria Acanthocheilonema viteae was shown to protect jirds against a challenge infection. Subcutaneous immunization with the protein reduced the number of adult worms by up to 65 % and the number of circulating microfilariae declined by up to 93 % in these animals. The protein is located in the muscle tissues of(More)
Jirds (Meriones unguiculatus) were immunized with irradiated (35 krad) stage-3 larvae (L3) of Acanthocheilonema viteae. The induced resistance against homologous challenge infection and the antibody response of the animals were studied. Immunization with 3, 2, or 1 dose of 50 irradiated L3 induced approximately 90% resistance. Immunization with a single(More)
The performance of indirect haemagglutination assays (IHA), enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescent antibody tests (IFAT) were compared with 450 sera from a Schistosoma mansoni-endemic area in Burkina Faso. All participants in this survey provided at least one sample each of stool, urine and serum. From those with an(More)
Eggs of different stages of development, egg membranes, and the fluid isolated from uterus and pseudocoelomic cavity show antigenic properties against sera from Wuchereriasis- and Onchocerciasis-patients in the indirect immunofluorescent test, while hatched larvae did not show any fluorescence. Isolation and simple drying of these antigenic materials on(More)
Using IFAT, it has been shown that isolated egg-shells and uterine fluid of Dipetalonema viteae are the most potent antigens in heterologous systems using human sera from patients infected with Wuchereria bancrofti, Onchocerca volvulus and Loa loa, as well as in homologous systems using sera from animals infected with D. viteae. It is suggested that these(More)
The role of oxidative stress resulting from production of reactive oxygen species and/or from suppression of the cellular antioxidant capacity in parasitic infections is shortly reviewed. The experimental part of the paper deals with the glutathione (GSH) — glutathione reductase (GR) system, a cornerstone of intracellular antioxidant defence mechanisms. For(More)
Immunofluorescence diagnosis of filariasis (sandwich technique) on histological sections ofDipetalonema viteae as antigen after fixation with buffered glutaraldehyde and embedding with methacrylate has certain advantages over the use of Cryostate sections: 1. the optical appearance of the immunofluorescence on the antigen is much better, the sections are(More)
The localization of the antigen-antibody reactions on methacrylate embedded sections of an adult male and female of the nematode Dipetalonema vitae by the indirect immunofluorescence test was found to be as follows: female D. vitae: in the egg-shell and circumference of sections of the microfilariae as well as amorphous structures in the vesica seminalis(More)