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To facilitate genetic studies in primary neurons, we analyzed the efficiency of cationic lipid-mediated plasmid DNA transfection using adherent and acutely dissociated neuronal suspensions derived from embryonic mouse cortical tissue. Compared to transfections using adherent cultures, the in-tube procedure enhanced the delivery of a GFP reporter plasmid(More)
An electrophysiologic technique for studying sensory fibers in the rat sural nerve is described. The conduction velocity of the sural nerve in rats is much slower than in humans, probably because of lack of large myelinated nerve fibers in rats. An experimental neuropathy (induced by hexachlorophene) that predominantly causes a myelin disorder produced a(More)
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