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Nerve sprouting in healed myocardial infarction has been associated with increased incidences of ventricular tachyarrhythmia and sudden cardiac death. However, the underlying electrophysiological mechanisms are unclear. To investigate the linkage between nerve sprouting and potassium channel function, we developed a rat model of cardiac sympathetic nerve(More)
Temporary hearing threshold shift (TTS) resulting from a “benign” noise exposure can cause irreversible auditory nerve afferent terminal damage and retraction. While hearing thresholds and acute tissue injury recover within 1–2 weeks after a noise overexposure, it is not clear if multiple TTS noise exposures would result in cumulative damage even though(More)
OBJECTIVES/HYPOTHESIS To compare three different inoculation techniques for the development of cytomegalovirus (CMV)-induced sensorineural hearing loss (SNHL) in a mouse model. STUDY DESIGN A prospective experimental animal study. METHODS BALB/c mice underwent inoculation using green fluorescent protein-expressing mouse cytomegalovirus (mCMV-GFP) via(More)
Jin Zhang, Chongyu Ren, Ling Chen, Manuel F. Navedo, Laura K. Antos, Stephen P. Kinsey, Takahiro Iwamoto, Kenneth D. Philipson, Michael I. Kotlikoff, Luis F. Santana, W. Gil Wier, Donald R. Matteson,* and Mordecai P. Blaustein* Departments of Physiology and Medicine, University of Maryland School of Medicine, Baltimore, Maryland; Department of Physiology(More)
The majority of auditory nerve fibers exhibit prominent spontaneous activity in the absence of sound. More than half of all auditory nerve fibers in CBA mice have spontaneous firing rates higher than 20 spikes/s, with some fibers exceeding 100 spikes/s. We tested whether and to what extent endbulb synapses are depressed by activity between 10 and 100 Hz,(More)
Usher syndrome type 2 (USH2) is the predominant form of USH, a leading genetic cause of combined deafness and blindness. PDZD7, a paralog of two USH causative genes, USH1C and USH2D (WHRN), was recently reported to be implicated in USH2 and non-syndromic deafness. It encodes a protein with multiple PDZ domains. To understand the biological function of PDZD7(More)
Mice with smooth muscle (SM)-specific knockout of Na(+)/Ca(2+) exchanger type-1 (NCX1(SM-/-)) and the NCX inhibitor, SEA0400, were used to study the physiological role of NCX1 in mouse mesenteric arteries. NCX1 protein expression was greatly reduced in arteries from NCX1(SM-/-) mice generated with Cre recombinase. Mean blood pressure (BP) was 6-10 mmHg(More)
Sympathetic denervation is frequently observed in heart disease. To investigate the linkage of sympathetic denervation and cardiac arrhythmia, we developed a rat model of chemical sympathectomy by subcutaneous injections of 6-hydroxydopamine (6-OHDA). Cardiac sympathetic innervation was visualized by means of a glyoxylic catecholaminergic histofluorescence(More)
L-type voltage-gated Ca(2+) channels (LVGCs) are functionally downregulated in arterial smooth muscle (SM) cells (ASMCs) of mice with SM-specific knockout of Na(+)/Ca(2+) exchanger type-1 (NCX1(SM-/-)) (32). Here, using activators and inhibitors of protein kinase C (PKC), we explore the regulation of these channels by a PKC-dependent mechanism. In both(More)
Chongyu Ren, Jin Zhang, Kenneth D. Philipson, Michael I. Kotlikoff, Mordecai P. Blaustein, and Donald R. Matteson Departments of Physiology and Medicine, University of Maryland School of Medicine, Baltimore, Maryland; Department of Physiology, University of California Los Angeles School of Medicine, Los Angeles, California; and Department of Biomedical(More)
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