Chin Nok Fong

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The effect of high K− concentration, insulin and the L-type Ca2− channel blocker PN 200-110 on cytosolic intracellular free calcium ([Ca2+]i) was studied in single ventricular myocytes of 10-day-old embryonic chick heart, 20-week-old human fetus and rabbit aorta (VSM) single cells using the Ca2+-sensitive fluorescent dye, Fura-2 microfluorometry and digital(More)
Endothelin-1 (ET-1) induced a concentration-dependent sustained increase of intracellular calcium ([Ca2+]i) in single cells of rabbit aortic vascular smooth-muscle cells (VSMCs). This sustained [Ca2+]i increase was insensitive to the L-type Ca2+ antagonist nifedipine but was sensitive to isradipine and the presence of extracellular Ca2+. The ET(A) receptor(More)
Intrafiber Na+-activity, (aNa)i, was measured in vivo in single muscle fibers in the gastrocnemius and soleus muscles of normal and dystrophic (dy2J/dy2J) C57BL/6J mice by means of double-barrel Na+-selective microelectrodes. Values of (aNa)i in normal gastrocnemius and soleus muscles were 9.8 +/- 1.0 mM (means +/- SE; N = 15) and 13.7 +/- 1 mM (N = 19),(More)
Chloride liquid ion-exchange microelectrodes were used to measure intracellular chloride activity (aCl)i or rabbit papillary muscle in vitro. In HCO3- -- CO2 buffered Ringer (aCl)i was 15.2 +/- 0.6 mM (n = 40) at pH 7.3 and 15.4 +/- 0.3 mM (n = 29) at pH 6.8. In HEPES-buffered Ringer (aCl)i was 24.1 +/- 0.6 mM (n = 21) at pH 7.3 and 20.0 +/- 0.3 mM (n = 17)(More)
Frog sartorius muscles were superfused for 40 min with solutions of K-free Ringer, normal Ringer containing ouabain, or K-free Ringer containing ouabain. Changes in myoplasmic K and Na were measured with ion-selective microelectrodes; changes in total fibre K and Na were measured by means of atomic absorption spectroscopy; and changes in total fibre water(More)
The effects of malnutrition on intracellular K+ activity, (alpha K)i, and membrane potential, Em, were measured by means of double-barrelled K+-selective microlectrodes in the soleus and gastrocnemius muscles of the rat. (alpha K)i and Em were measured in vivo in normal anaesthetized animals and in rats subjected to one of two diet restrictions: a 2-day(More)
Ca2+ buffering in axoplasm extruded from the squid giant axon was studied by axoplasmic dilution and Ca2+-selective electrodes. ATP-dependent Ca2+ buffering was lost when metabolism and endogenous ATP production were inhibited by axoplasmic dilution. Under these conditions ('low ATP' axoplasmic suspensions) Ca2+ activity (aCa) increased spontaneously. This(More)
Intracellular potassium activity, (aK)i, and axoplasmic K+ concentration, [K+]i, were measured by means of K+-selective microelectrodes and atomic absorption spectroscopy, respectively, in squid giant axons dialyzed with K+-free dialysis solution and bathed in K+-free artificial sea water. (aK)i measurements indicated that axoplasmic free K+ could be(More)
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