Damaged or mismatched bases in DNA can be repaired by base excision repair enzymes (BER) that replace the defective base. Although the detailed molecular structures of many BER enzymes are known, how they colocalize to lesions remains unclear. One hypothesis involves charge transport (CT) along DNA [Yavin et al., Proc. Natl. Acad. Sci. U.S.A. 102, 3546… (More)
Mechanical force is present in all aspects of living systems. It affects the conformation of molecules, the shape of cells, and the morphology of tissues. All of these are crucial in architecture-dependent biological functions. Nanoscience of advanced materials has provided knowledge and techniques that can be used to understand how mechanical force is… (More)
Budding yeast cells exist in two mating types, a and α, which use peptide pheromones to communicate with each other during mating. Mating depends on the ability of cells to polarize up pheromone gradients, but cells also respond to spatially uniform fields of pheromone by polarizing along a single axis. We used quantitative measurements of the response of a… (More)
1 Computational Methods 1.1 Model description We assume that Ω = B(O, R) \ B(O, R n) describes the cell, thus the cell periphery is the circle of radius R. The cytoplasmic Cdc42 density is denoted by n(t, x), the membrane density of Cdc42 is denoted µ(t, s) and the density of cytoskeletal tracks is denoted by c(t, x). Let us recall the model ∂ t n(t,… (More)
We present a wide-field multi-photon microscopy that provides optical sectioning at high frame rate under biocompatible laser dosage. Axial resolution comparable to confocal microscopy and 5-frame-per-second live tissue imaging are demonstrated.