Chikara Miyazaki

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To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin(More)
To develop an efficient gene tagging system in rice, a plasmid was constructed carrying a non-autonomous maize Ds element in the untranslated leader sequence of a hygromycin B resistance gene fused with the 35S promoter of cauliflower mosaic virus. This plasmid was cotransfected by electroporation into rice protoplasts together with a plasmid containing the(More)
At least ten polypeptides larger than 6 kilodaltons (K) are produced in minicells from the miniplasmid pSM1 in vivo. pSM1 (5804 bp) is a small derivative of the drug resistance plasmid R100 (ca. 90 kb) and carries the R100 essential replication region as well as some non-essential functions. Cloned restriction fragments of pSM1 and plasmids with deletions(More)
We have been studying the characteristics of the sequence generated by the logistic map over integers. In this paper, we examine the relationship of the NIST statistical tests to the Lyapunov exponents of the sequence. Then, we confirm good correlations between a sign of the exponents and both p-value and proportion of the NIST statistical tests. Therefore,(More)
We are interested in the properties of sequences generated by the logistic map over integers. In the present paper, we give the Lyapunov exponents of the sequences generated by the map. Then, we confirm good correlations between a sign of the exponents and a few properties of randomness such as averages periods and link-lengths, and an occurrence rate of(More)
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