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An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effects. With the new, M-9, medium produced(More)
Shikonin derivatives were produced from cell suspension cultures of Lithospermum erythrorhizon for the first time. The results of studies on various culture media showed that the type of nitrogen source in the medium greatly influenced the production of these derivatives. When nitrate was the nitrogen source, stable production of shikonin derivatives by the(More)
A method for quantitative analysis of shikonin derivatives using high pressure liquid chromatography (HPLC) was established. With this method the composition of shikonin derivatives in cultured cells and roots of Lithospermum erythrorhizon (ko-shikon) was compared. The composition of shikonin derivatives produced by cell suspension cultures was similar to(More)
A two-layer culture method was established that uses an organic solvent to remove shikonin derivatives produced on cell surfaces during the culture of suspension cells of Lithospermum erythrorhizon. Some paraffins and a fatty acid ester made suitable solvents, whereas olefins and aromatic solvents extensively inhibited the production of shikonin(More)
The present study was carried out to evaluate an equivalence of pharmacological properties between natural crude drugs and their cultured cells. The effects of ether extract of Lithospermi Radix and cultured cells of Lithospermum erythrorhizon Sieb. et Zucc. and aqueous extract of Coptidis Rizoma and cultured cells of Coptis japonica Makino var. dissecta(More)
This paper describes the development toolkit for persuasive applications. We developed the programmable USB-driven gumball-dispenser tool, as the platform for environmental sensing and delivering physical feedback to the users. The application for NASA Sustainability Base, LEEDS platinum building was developed based on the toolkit, to motivate people to(More)
This study presents the preliminary attempt to investigate the usage of driver physiology signals, including electrocardiography (ECG) and respiration wave signals, to predict driving maneuvers. While most studies on driving maneuver prediction uses direct measurements from vehicle or road scene, we believe the mental state changes from the driver when(More)
Drivers' inattention to obstacles on the road is a major cause of traffic accidents, and developing advanced driver assistance system (ADAS) to support the driver's visual search has been a challenge. This is partly due to the complexity of developing user interfaces that indicate the positional information of objects around the vehicle, such as pedestrians(More)
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