Chiga Hayashi

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Cells with many of the features of mast cells develop in cultures of normal mouse hematopoietic cells containing mast cell growth factor (1-8), an activity similar or identical to interleukin 3 (IL-3) ~ (9-11), histamine-producing cell-stimulating factor (9), or P cell-stimulating factor (12-14). Large numbers of these cells (>10 s) can be generated as(More)
In this study, we investigated the role of store-operated Ca2+ channels (SOCC) on ACTH release using microperifusion system. The SOCC blockers, SKF96365 and MRS1845, did not affect the ACTH response to single AVP stimulation. After the depletion of intracellular Ca2+ stores by treating with ionomycin, SOCC blockers reduced the initial spike phase of ACTH(More)
Neuropeptide W (NPW) was isolated as an endogenous ligand for NPBWR1, an orphan G protein-coupled receptor localized in the rat brain, including the paraventricular nucleus. It has been reported that central administration of NPW stimulates corticosterone secretion in rats. We hypothesized that NPW activates the hypothalamic-pituitary-adrenal (HPA) axis via(More)
The present study investigated the role of K(+) channels in the inhibitory effect of glucocorticoid on adrenocorticotropin (ACTH) release induced by corticotropin-releasing hormone (CRH) using cultured rat anterior pituitary cells. Apamin and charybdotoxin (CTX) did not have a significant effect on ACTH release induced by CRH (1 nM). Tetraethylammonium(More)
Peritoneal mast cells and macrophages are commonly used to study functions of these two types of cells (1-3). Although many investigators have studied the production of peritoneal macrophages both in vivo (4-6) and in vitro (7, 8), only a few investigators have taken an interest in production of peritoneal mast cells (9-11). Both mast cells and macrophages(More)
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