Chieri Kubota

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Leafy or chlorophyllous explants of a number of plant species currently micropropagated have been found to have high photosynthetic ability. Their growth and development have been promoted on sugar-free medium rather than on sugar-containing medium, provided that the environmental factors, such as CO2 concentration, light intensity and relative humidity,(More)
Cherries, and in particular sweet cherries, are a nutritionally dense food rich in anthocyanins, quercetin, hydroxycinnamates, potassium, fiber, vitamin C, carotenoids, and melatonin. UV concentration, degree of ripeness, postharvest storage conditions, and processing, each can significantly alter the amounts of nutrients and bioactive components. These(More)
including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is due not to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have(More)
The effect of exogenous methyl jasmonate (MeJA) on antioxidative compounds of romaine lettuce ( Lactuca sativa L.) was investigated. Lettuces were treated with various MeJA solutions (0, 0.05, 0.1, 0.25, and 0.5 mM) before harvest. Total phenolic compounds content and antioxidant capacity of romaine lettuce significantly increased after MeJA treatments(More)
The growth and net photosynthetic rates of shoot cultures of Eucalyptus tereticornis Smith grown under various photoautotrophic conditions (without sucrose in the nutrient medium and with enriched CO2 and high photosynthetic photon flux or PPF) were compared with those of shoot cultures grown under conventional photomixotrophic conditions (30 g l−1 sucrose(More)
Potato plantlets (Solanum tuberosum L. cv. Benimaru) were cultured under photoautotrophic (without any sucrose in the nutrient medium and with enriched CO2 and high photosynthetic photon flux) and photomixotrophic conditions (20 g 1−1 sucrose in the medium). Leaf anatomy and stomatal characteristics of the leaves were studied in relation to stomatal size(More)
A forced ventilation system has been developed for large-scale photoautotrophic micropropagation of chlorophyllous plants. The major goal of the system is to provide a uniform supply of CO2 inside a large culture vessel (volume 3480 ml) to achieve uniform growth of the plantlets. The system has been designed such that sterile nutrient solution can be(More)
The influence of salinity stress on the growth, appearance, and nutritional compounds, especially phenolic compounds and carotenoids, of romaine lettuce (Lactuca sativa L.), a low salt tolerant plant, was studied. The dry weight, height, and color of the lettuce plants were significantly changed by long-term irrigation (15 days) with higher NaCl(More)
The growth and water relations of Paulownia fortunei in photoautotrophic cultures (nutrient medium lacking sucrose and growth regulator) with CO2 enrichment (PWAH) or without CO2 enrichment (PWAL) were compared with those in photomixotrophic shoot (PWC; 30 g dm−3 sucrose and 0.3 mg dm−3 N6-benzyladenine) and root cultures (PWR; 0.3 mg dm−3 indole-3-butyric(More)
In order to characterize environmental variables inside a plant canopy under artificial lighting in the CELSS, we investigated the effects of light intensity and air velocity on air temperature, water vapor pressure, and CO2 concentration inside a plant canopy. Under a PPF of 500 micromoles m-2 s-1, air temperature was 2-3 degrees C higher, water vapor(More)