Chia Ho Cheng

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Freezing avoidance conferred by different types of antifreeze proteins in various polar and subpolar fishes represents a remarkable example of cold adaptation, but how these unique proteins arose is unknown. We have found that the antifreeze glycoproteins (AFGPs) of the predominant Antarctic fish taxon, the notothenioids, evolved from a pancreatic(More)
1. The biological activities of the proteinase-activated receptor number 2 (PAR-2)-derived peptides, SLIGRL (PP6) SLIGRL-NH2 (PP6-NH2) and SLIGR-NH2 (PP5-NH2) were measured in mouse and rat gastric longitudinal muscle (LM) tissue and in a rat aortic ring preparation and the actions of the PAR-2-derived peptides were compared with trypsin and with the(More)
In this study two aerobic phthalic acid ester (PAE) degrading bacteria strains, DK4 and O18, were isolated from river sediment and petrochemical sludge, respectively. The two strains were found to rapidly degrade PAE with shorter alkyl-chains such diethyl phthalate (DEP), dipropyl phthalate (DPrP), di-n-butyl phthalate (DBP), benzylbutyl phthalate (BBP) and(More)
Antarctic notothenioid fishes and several northern cods are phylogenetically distant (in different orders and superorders), yet produce near-identical antifreeze glycoproteins (AFGPs) to survive in their respective freezing environments. AFGPs in both fishes are made as a family of discretely sized polymers composed of a simple glycotripeptide monomeric(More)
Three protoberberine alkaloids jatrorrhizine, berberine and palmatine were isolated from the monoamine oxidase (MAO) inhibitory fraction of the methanol extract of Coptis chinensis rhizoma. Jatrorrhizine was shown to inhibit non-competitively both MAO-A and -B from rat brain mitochondria with the IC50 values of 4 and 62 microM, respectively. Berberine only(More)
Type III antifreeze protein (AFP) in the Antarctic eel pout (Lycodichthys dearborni) occurs as a heterogeneous family of three major and at least five minor variants collectively maintained at high year-round blood levels (> 20 mg/ml). Two major AFPs (RD1, RD2) are 7 kD in size, and the third (RD3) is 14 kD and is composed of two 7-kD AFP domains linked by(More)
Stoneley waves in permeable boreholes are diagnostic of formation permeability because their propagation is affected by the dynamic fluid flow at the borehole wall. We characterize this flow using the concept of dynamic permeability. We examined the applicability of the dynamic permeability to porous media by applying it to a single fracture case and found(More)
C(60), vitamin E, and three C(60) derivatives (polar 1 and water-soluble C(3)/D(3)C(60)s) were examined for their antioxidant effects on prevention of lipid peroxidation induced by superoxide and hydroxyl radicals. The protection effect on lipid peroxidation was found to be in the sequence: C(60) >/= vitamin E > 1 > none, for liposoluble antioxidants, and(More)
The structural heterogeneity of the major antifreeze peptides (AFPs) from the antarctic eel pout, Lycodichthys dearborni (formerly classified as Rhigophila dearborni) was characterized. Three major AFPs designated as RD1, RD2 and RD3, and five minor ones were isolated from the fish plasma. RD1 and RD2 are both 64 residues in length, about 7 kDa, and thus(More)
To verify the role of porcine reproductive and respiratory syndrome virus (PRRSV) infection on pulmonary defense mechanisms, alterations in the viability, morphology, and various functions of porcine alveolar macrophages (AMs) were evaluated in vitro for 2-72 h after exposure to a Taiwan isolate, tw91, at a multiplicity of infection (MOI) of 0.1. A low but(More)