Cheryl Shaffer

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Amiloride and four analogues of amiloride were shown to inhibit Na+-dependent, phlorizin-sensitive hexose uptake by a clone of pig kidney cells, LLC-PK1/Cl4. The analogues tested were: 5-(N-ethyl-N-isopropyl)amiloride (EIPA), 5-(N-methyl-N-isobutyl)amiloride (MIBA), 3',4'-dichlorobenzamil, and phenamil. The transport substrate was the nonmetabolizable(More)
Cultured pig kidney cells designated LLC-PK1, previously shown to acquire Na+-dependent concentrative transport of hexoses as the cells become growth arrested, also show Na+-dependent concentrative uptake of the amino acid analogs alpha-aminoisobutyric acid (AIB) and (methyl) meAIB. This A system-like transport is most active in sparse, growing cultures and(More)
[3H]Ouabain specifically bound at sublethal concentrations to Na,K-ATPase on the surface of HeLa cells is taken up (internalized) by the cells at a rate of three membrane equivalents of labeled sites per generation. Immediately following a pulse label with the glycoside, codistribution of radioactivity with the surface marker 5'-nucleotidase is found in(More)
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