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Role of an archaeal PitA transporter in the copper and arsenic resistance of Metallosphaera sedula, an extreme thermoacidophile.

• Samuel McCarthy, Chenbing Ai, +4 authors Paul Blum
• Journal of bacteriology
• 2014

Thermoacidophilic archaea, such as Metallosphaera sedula, are lithoautotrophs that occupy metal-rich environments. In previous studies, an M. sedula mutant lacking the primary copper efflux… (More)

Expression, Purification and Molecular Structure Modeling of Thioredoxin (Trx) and Thioredoxin Reductase (TrxR) from Acidithiobacillus ferrooxidans

• Yiping Wang, Xiaojian Zhang, Qing Liu, Chenbing Ai, Hongyu Mo, Jia Zeng
• Current Microbiology
• 2009

The thioredoxin system consists of thioredoxin (Trx), thioredoxin reductase (TrxR) and NADPH, which plays several key roles in maintaining the redox environment of the cell. In Acidithiobacillus… (More)

Enhancement of Metallosphaera sedula Bioleaching by Targeted Recombination and Adaptive Laboratory Evolution.

• Samuel McCarthy, Chenbing Ai, Paul Blum
• Advances in applied microbiology
• 2018

Thermophilic and lithoautotrophic archaea such as Metallosphaera sedula occupy acidic, metal-rich environments and are used in biomining processes. Biotechnological approaches could accelerate these… (More)

Glu43 is an essential residue for coordinating the [Fe2S2] cluster of IscR from Acidithiobacillus ferrooxidans.

• Jia Zeng, Xiaojian Zhang, Yiping Wang, Chenbing Ai, Qing Liu, Guanzhou Qiu
• FEBS letters
• 2008

The iron-sulfur cluster regulator (IscR) was reported to be a repressor of the iscRSUA operon. In vitro transcription reactions revealed that the IscR had a repression effect on the iscR promoter.… (More)

Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution

• Chenbing Ai, Samuel McCarthy, Valerie Eckrich, Deepak Rudrappa, Guanzhou Qiu, Paul Blum
• Journal of Industrial Microbiology…
• 2016

Extremely thermoacidophilic members of the Archaea such as the lithoautotroph, Metallosphaera sedula, are among the most acid resistant forms of life and are of great relevance in bioleaching. Here,… (More)

Complete Genome Sequences of Evolved Arsenate-Resistant Metallosphaera sedula Strains

• Chenbing Ai, Samuel McCarthy, Wendy S. Schackwitz, Joel Martin, Anna Lipzen, Paul Blum
• Genome announcements
• 2015

Metallosphaera sedula is a thermoacidophilic crenarchaeote with a 2.19-Mb genome. Here, we report the genome sequences of several evolved derivatives of M. sedula generated through adaptive… (More)

Identification and Analysis of a Novel Gene Cluster Involves in Fe2+ Oxidation in Acidithiobacillus ferrooxidans ATCC 23270, a Typical Biomining Acidophile

• Chenbing Ai, Yuting Liang, Bo Miao, Miao Chen, Weimin Zeng, Guanzhou Qiu
• Current Microbiology
• 2018

Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for… (More)

Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula

• Chenbing Ai, Samuel McCarthy, Yuting Liang, Deepak Rudrappa, Guanzhou Qiu, Paul Blum
• Journal of Industrial Microbiology…
• 2017

Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula… (More)

Expression, Purification, and Characterization of an Iron Chaperon Protein CyaY from Acidithiobacillus ferrooxidans

• Chenbing Ai, Hongyu Mo, +4 authors Jia Zeng
• Current Microbiology
• 2010

CyaY is the bacterial homolog of frataxin, proposed to be involved in the assembly of iron–sulfur clusters. While, the physiological iron donor for the iron–sulfur clusters assembly remains… (More)

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