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The present study describes the response of amoeboid and ramified microglial cells in the corpus callosum to intraperitoneal lipopolysaccharide injections in postnatal rats as examined by lectin histochemical staining and electron microscopy. In 1 day old rats receiving endotoxin injections and sacrificed at various time intervals, the lectin labelling of(More)
The morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (E21) and 15 d postnatally are described. Using lectin labelling, 5 morphological types of labelled cells (R, SP, KLP, TLP, AP) based on cell body shape, the(More)
The distribution of various morphological forms of microglia stained with lectin in different regions of postnatal rat brain was examined in 2 planes of section, coronal and sagittal. In the coronal sections (mediolateral plane) taken at the level of the optic chiasma, the lectin-labelled cells were examined in the gustatory (GU), lateral somatosensory(More)
Lipopolysaccharide (LPS), the major proinflammatory component of gram-negative bacteria, is well known to induce sepsis and microglial activation in the CNS. On the contrary, the effect of products from gram-positive bacteria especially in areas devoid of blood-brain barrier remains to be explored. In the present study, a panel of antibodies, namely, OX-6,(More)
The present study described an ultrastructural synaptic configurations between primary afferent terminals (PATs), cuneothalamic relay neurons (CTNs) and GABA-immunoreactive (GABA-IR) boutons in the cuneate nucleus of rats using cervicothoracic dorsal rhizotomies, retrograde transport of wheat germ agglutinin conjugated with horseradish peroxidase complex(More)
Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunohistochemistry have been used to characterize the nitric oxide (NO)-containing neurons in the rat cuneate nucleus. The present results showed that NADPH-d-positive/NOS-immunoreactive (-IR) neurons were distributed in the entire rostrocaudal(More)
This study was aimed to investigate the possible involvement of neurons in the cuneate nucleus (CN) in the processing of A beta afferent inputs evoked by electrical stimulation of constricted median nerve in rats with behavioral signs of neuropathic pain. Immunohistochemical localization of Fos protein was used to examine the neuronal activation, and the(More)
This study was aimed to clarify whether the primary afferent terminals (PATs), GABAergic terminals, and glutamatergic terminals made direct synaptic contacts with glycine-IR neurons in the cuneate nucleus of rats. In this connection, injection of the anterograde tracer WGA-HRP into brachial plexus, antiglycine preembedding immunoperoxidase, and anti-GABA,(More)
Using anterograde transport of WGA-HRP and the experimental degeneration method for identification of corticocuneate (CCT) and primary afferent (PAT) terminals in conjunction with gamma-amino butyric acid (GABA) and glutamate immunocytochemistry, this study has demonstrated that the GABA-immunoreactive (GABA-IR) neurons in the rat cuneate nucleus were(More)
To determine why a vaginal self-collection tested for high-risk human papillomavirus (HR-HPV) by Hybrid Capture 2(R) (hc2) has lower sensitivity and specificity for cervical intraepithelial neoplasia Grade 2 or worse (> or = CIN 2), we collected 5 specimens (endocervix, upper and lower vagina, perineum, vaginal self-collection) from 2,625 women.(More)