Chen Hong-shan

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OBJECTIVE To establish fluorescence resonance energy transfer (FRET) assay method of detecting proteolytic activity of non-structural protein 3-4A (NS3-4A) serine protease of hepatitis C virus (HCV) for high throughput screening inhibitors against HCV in vitro. METHODS HCV recombinant plasmid pMAL~c2/NS3-4A was transformed into the E.coli strain K12TB1.(More)
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