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A unique transposon was found in the chromosome of Bacillus megaterium MB1, a Gram-positive bacterium isolated from mercury-polluted sediments of Minamata Bay, Japan. The transposon region of a 14.5kb DNA fragment was amplified by PCR using a single PCR primer designed from the nucleotide sequence of an inverted repeat of class II transposons. The molecular(More)
The complete structure of a broad-spectrum mercury resistance module was shown by sequencing the Gram-positive bacterial transposon TnMERI1 of Bacillus megaterium MB1. The regions encoding organomercury resistance were identified. Upstream of a previously identified organomercurial lyase merB (merB1) region of TnMERI1, a second merR (merR2) and a second(More)
A 3.6-kbp DNA fragment was cloned from the extrachromosomal DNA of a pathogenic plant mollicute, onion yellows phytoplasma (OY-W). Sequence analysis of the fragment revealed an open reading frame (ORF) encoding the replication (Rep) protein of rolling-circle replication (RCR)-type plasmids. This result suggests the existence of a plasmid (pOYW1) in OY-W(More)
Forensic voice comparison (FVC) systems have often involved manual annotation of usable phonetic units, requiring substantial human labor. Recent research has shown the efficacy of automatic methods in FVC, and this paper investigates automatic phonetic segmentation in FVC systems. Nasals and vowels were found to contribute the most in terms of improvements(More)
BACKGROUND Although fecal occult blood test and invasive endoscopic examination are common used to detect colorectal adenomas and cancers, non-invasive and specific biomarkers are still under investigation. The objective is to evaluate the biomarker CYP1B1 alone or in combination with aryl hydrocarbon receptor (AhR), nuclear beta-catenin, p53 or bcl-2 for(More)
Automatic forensic voice comparison (FVC) studies have often employed Gaussian Mixture Model - Universal Background Model (GMM-UBM) modeling based on mean-only maximum a posteriori (MAP) adaptation or full MAP adaptation with little consideration of other variants of MAP adapted configurations. Our study indicates that an FVC system improvement in(More)
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