Charlotte Nini Gudme

Learn More
The inhibitory 2DL1 and activating 2DS1 killer Ig-like receptors (KIR) both have shared ligand specificity for codon sequences in the C2 group HLA-Cw Ags. In this study, we have investigated NK cell activation by allogeneic target cells expressing different combinations of the HLA-KIR ligand groups C1, C2, and Bw4. We demonstrate that fresh NK cells as well(More)
Frequent infusions of intravenous factor VIII (FVIII) are required to prevent bleeding associated with hemophilia A. To reduce the treatment burden, recombinant FVIII with a longer half-life was developed without changing the protein structure. FVIII-polyethylene glycol (PEG) conjugates were prepared using an enzymatic process coupling PEG (ranging from 10(More)
The primary secretion formed in various exocrine glands has a [K+] 2–5 times that of plasma. In this study we measured the transepithelial flux of 36Cl–, 22Na+ and 42K+ across the frog skin and applied the single-channel patch-clamp technique to the apical membrane of frog skin gland acini to investigate the pathway taken by K+ secreted by the glands.(More)
Immunogenicity is an important consideration in the licensure of a therapeutic protein because the development of neutralizing anti-drug antibodies (ADAs) can affect both safety and efficacy. Neoantigens introduced by bioengineering of a protein drug are a particular cause for concern. The development of a bioengineered recombinant factor VIIa (rFVIIa)(More)
In the present work, the effect of stimulation of alpha-adrenergic receptors on Cl- secretion via exocrine frog skin glands was investigated. The alpha-adrenergic stimulation was performed by addition of the adrenergic agonist noradrenaline in the presence of the beta-adrenergic antagonist propranolol. In the presence of propranolol, noradrenaline had no(More)
INTRODUCTION Progressive arthropathy caused by recurrent joint bleeds is a severe complication in haemophilia. AIM We investigated whether biomarkers of cartilage and bone degradation, and inflammation were altered in haemophilia patients and whether these biomarkers could identify haemophilia patients with arthropathy. METHODS Serum from 35 haemophilia(More)
UNLABELLED Essentials Von Willebrand factor (VWF) stabilizes factor VIII (FVIII) and prevents its premature clearance. Rat anatomical and hepatocellular distribution studies assessed the VWF effect on FVIII clearance. Hepatocytes and liver sinusoidal endothelial cells play a key role in FVIII clearance. Anatomical and hepatocellular distribution of FVIII is(More)
Henning R. Stennicke, Marianne Kjalke, Ditte M. Karpf, Kristoffer W. Balling, Peter B. Johansen, Torben Elm, Kristine Øvlisen, Flemming Möller, Heidi L. Holmberg, Charlotte N. Gudme, Egon Persson, Ida Hilden, Hermann Pelzer, Henrik Rahbek-Nielsen, Christina Jespersgaard, Are Bogsnes, Anette A. Pedersen, Anne K. Kristensen, Bernd Peschke, Wendy Kappers,(More)
In the present study we examined the effect of α-adrenergic regulation of active transepithelial Na+ absorption across the isolated frog skin epithelium. α-Adrenergic stimulation was achieved by addition of the adrenergic agonist noradrenaline in the presence of the β-adrenergic blocker propranolol. α-Adrenergic stimulation inhibited basal as well as(More)
  • 1