Charleston Noble

Tobias Ambjörnsson4
Joachim Fritzsche3
Fredrik Westerlund3
Adam N. Nilsson3
4Tobias Ambjörnsson
3Joachim Fritzsche
3Fredrik Westerlund
3Adam N. Nilsson
2Jonas O. Tegenfeldt
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We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidic channels. We optimize the experimental(More)
  • Mohammadreza Alizadehheidari, Erik Werner, Charleston Noble, Michaela Reiter-Schad, Lena K Nyberg, Joachim Fritzsche +5 others
  • 2016
Studies of circular DNA confined to nanofluidic channels are relevant both from a fundamental polymer-physics perspective and due to the importance of circular DNA molecules in vivo. We here observe the unfolding of DNA from the circular to linear configuration as a light-induced double strand break occurs, characterize the dynamics, and compare the(More)
The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of(More)
  • Charleston Noble, Adam N. Nilsson, Camilla Freitag, Jason P. Beech, Jonas O. Tegenfeldt, Tobias Ambjörnsson
  • 2015
Optical mapping by direct visualization of individual DNA molecules, stretched in nanochannels with sequence-specific fluorescent labeling, represents a promising tool for disease diagnostics and genomics. An important challenge for this technique is thermal motion of the DNA as it undergoes imaging; this blurs fluorescent patterns along the DNA and results(More)
One of the key challenges in modeling the dynamics of contagion phenomena is to understand how the structure of social interactions shapes the time course of a disease. Complex network theory has provided significant advances in this context. However, awareness of an epidemic in a population typically yields behavioral changes that correspond to changes in(More)
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