Charles W. Holliday

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Silver staining of the adult brine shrimp, Artemia salina, revealed that only the metepipodites of the phyllopodia were significantly permeable to chloride and/or silver ions. The metepipodites stained in a reticulated pattern, possibly indicating areas in the cuticle over cells specialized for chloride secretion. Crude homogenates of metepipodites had very(More)
We are engaged in a comprehensive study of cicada killers (Sphecius spp.), including their behavioral ecology. At one location, we observed interactions among three putative species of Sphecius, and used DNA barcoding to help clarify relationships among them. For this, we sequenced a fragment of the mitochondrial cytochrome c oxidase subunit I gene. During(More)
The characteristics of successfully mating male cicada killers (Sphecius speciosus Drury) were compared to those of unsuccessful males. In the field, successful males were larger and had higher ratios of flight muscle mass to body mass (flight muscle ratios) than unsuccessful males. The elevated flight muscle ratio of successful males was not because of(More)
Crab urinary bladder appears to possess several morphological and functional similarities to vertebrate renal proximal tubule. Sections of intermolt rock crab bladder accumulated PAH by a process that was concentrative (60 min tissue-to-medium ratio (T/M) for 10 microM PAH averaged 24), Na dependent, powered by glycolytic metabolism, and inhibitable by(More)
Uptake of 10 microM p-aminohippuric acid (PAH) by sections of Cancer borealis urinary bladder was concentrative, saturable (Km 67 microM, Vmax 1.7 tissue-1.h-1), inhibitable by other organic anions, and dependent on medium Na and glycolytic metabolism. Bladders mounted in flux chambers exhibited net secretory transport of PAH, with serosa-to-lumen(More)
In the crab, Cancer borealis, initial clearance studies showed a potent renal excretory system for the model organic cation, tetraethylammonium (TEA). TEA clearance averaged 145 +/- 32 ml/day, which was 18 times the paired polyethylene glycol clearance. TEA uptake by slices of urinary bladder was concentrative, saturable, inhibitable by(More)
To determine the characteristics of p-aminohippuric acid (PAH) excretion in Cancer irroratus and C. borealis, crabs were given a single injection of radiolabeled PAH and polyethylene glycol (PEG), and serum, urine, tissue, and seawater concentrations of each were measured over the next 6 days. In both species, PEG clearances averaged 5-10 ml serum/day;(More)
To characterize the organic anion transport properties of the luminal (1) and serosal (s) membranes of crab urinary bladder, initial (10 min) fluxes (J) and tissue accumulations (Ac) of 10 microM PAH were measured in the absence and presence of 1 mM BCG (nontransported, high-affinity competitor). Control bladders exhibited net reabsorptive transport with a(More)
The distributions of American cicada killers (Sphecius) were examined via solicited and museum specimens. S. hogardii occurs in southern Florida and several Caribbean islands. S. speciosus occurs throughout the east coast, southeast, and Midwestern states at high densities, and its range extends much farther west than expected, but not west of the(More)