Chao-Ming Mao

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AIM The diagnostic role of carcinoembryonic antigen (CEA), squamous cell carcinoma (SCC) antigen, Cyfra 21-1 and neuron-specific enolase (NSE) in the bronchoalveolar lavage fluid (BALF) for lung cancer is still controversial. The aim of this study was to evaluate the diagnostic value of these four tumor markers in BALF for peripheral lung cancer. METHODS(More)
This study was aimed to explore the effect of arsenic trioxide (ATO) on proliferation and apoptosis of mantle cell lymphoma (MCL) cell lines and the underlying mechanisms of the apoptosis. MCL cell lines (jeko-1, mino, JVM-2) were treated with different concentrations of ATO, then growth profile of these cells were detected by MTT. Apoptosis of ATO-treated(More)
AIM To express and purify the extracellular region of human glucocorticoid-induced tumor necrosis factor (hGITR(aa27-165)), and to prepare and identify the polyclonal antibody (pAb) against this fusion protein. METHODS The 429 bp DNA sequence of hGITR(aa27-165) was obtained from pGEM T-hGITR by PCR and then it was inserted into pQE30 plasmid to construct(More)
AIM To explore the effect of lincomycin (lin) on the immune function of dendritic cell (DCs) line DC2.4. METHODS Three experimental groups, namely, DC2.4 cells group, DC2.4 Cells+LPS group and DC2.4 cells +LPS +lin group were established (LPS and lin were 500 ng/mL). The morphological changes in each group were observed under inverted microscope. The MHC(More)
Glucocorticoid induced tumor necrosis factor receptor ligand (GITRL) is a type II transmembrane protein with homology to TNF receptor superfamily members, and its human receptor (hGITR), an ortholog of the recently discovered murine glucocorticoid-induced TNFR-related (mGITR) protein. Ligation of murine GITR/GITRL can enhance immunity reactions via several(More)
AIM To construct the recombination adeno-associated virus (rAAV) carrying murine forkhead box P3 (FoxP3) gene, and then detect the expression in NIH3T3 cells. METHODS The recombination adeno-associated virus (rAAV) vector containing internal ribosome entry site which could coordinate the expression of FoxP3 gene and enhance green fluorescent protein gene(More)
AIM To prepare anti-c-Kit monoclonal antibodies and characterize their specificity of epitope recognition. METHODS cDNA encoding human c-Kit extracellular domain was constructed into a procaryotic expression vector pQE30 and the correctness of the reconstructed plasmid pQE30-KitD4-5 was verified by sequencing. The plasmid was transformed into E.coli M15(More)
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