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—Although functional coupling between protein kinase C⑀ (PKC⑀) and mitochondria has been implicated in the genesis of cardioprotection, the signal transduction mechanisms that enable this link and the identities of the mitochondrial proteins modulated by PKC⑀ remain unknown. Based on recent evidence that the mitochondrial permeability transition pore may be(More)
Technical Appendix Table 1. Current farms status in this study, China Farm No. sows Vaccination* Illness rate ,%/y Mortality rate. % YL 1,000 Yes 90 50 HS 1,400 No 100 80 CY 300 Yes 80 90 SW 1,000 Yes 80 100 DY 2,000 Yes 95 100 JL 80 No 75 80 ZJ 90 No 100 90 MZ 100 No 60 100 *Sows were vaccinated with divalent inactivated transmissible gastroenteritis (TGE)(More)
Since late 2010, the outbreak of porcine epidemic diarrhea (PED) in China has resulted in the deaths of millions of suckling piglets. The main cause of the disease outbreak was unknown. In this study, partial spike (S), ORF3, and membrane (M) genes amplified from these variants were sequenced and analyzed. The results showed that the variants could be(More)
A novel multiplex detection array based on Luminex xMAP technology was developed and validated for simultaneous detection of five major viruses causing swine reproductive diseases. By combining one-step asymmetric multiplex reverse transcription polymerase chain reaction (RT-PCR) with xMAP bead-based hybridization and flow cytometry analysis, the resulting(More)
Porcine reproductive and respiratory syndrome virus (PRRSV), and particularly its highly pathogenic genotype (HP-PRRSV), have caused massive economic losses to the global swine industry. To rapidly identify HP-PRRSV, we developed a direct real-time reverse transcription polymerase chain reaction method (dRT-PCR) that could detect the virus from serum(More)
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