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Effects of cryopreservation on Ca2+ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa.
- Joon-Chul Kim, Yuhua Li, Sunwoo Lee, Y. Yi, Chang-Sik Park, S. Woo
- Biology, Medicine
- Molecules and cells
- 31 December 2008
Cryopreservation reduces the responsiveness of spermatozoa to depolarization, modulators of the internal Ca2+ store and progesterone in terms of the Ca2+. Expand
Early embryonic lethality caused by targeted disruption of the TRAF-interacting protein (TRIP) gene.
- Eui-Soon Park, Seunga Choi, +5 authors J. Rho
- Biology, Medicine
- Biochemical and biophysical research…
- 30 November 2007
Results indicate that TRIP is an essential factor during early mouse embryonic development in vivo, and this work has generated TRIP-deficient mice that were found to die shortly after implantation due to proliferation defects and excessive cell death. Expand
Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization1
It is concluded that sperm-acrosomal UCHs are involved in sperm-ZP interactions and antipolyspermy defense and could facilitate the management of polysPermy during IVF and provide insights into male infertility. Expand
Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer.
- Seongkeun Cho, K. Hwang, +5 authors Jin-Hoi Kim
- Biology, Medicine
- The Journal of reproduction and development
- 1 April 2009
The production of transgenic pigs using somatic cell nuclear transfer and a technique for removing nontransfected donor cells and for creating different founder animals has not yet been fully elucidated provide a valuable experimental model for applying and evaluating transgenic technology in pigs. Expand
Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal… Expand
Inhibition of 19S Proteasomal Regulatory Complex Subunit PSMD8 Increases Polyspermy During Porcine Fertilization In Vitro.
Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17째C and 4째C, and Frozen-thawed Semen
It was found out that the original quality of boar semen with cold shock resistance sperm played an important role and the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. Expand
46 DIFFERENTIAL PATTERNS OF PROTEIN SYNTHESIS BETWEEN NORMAL AND CLONED PLACENTAE
Practical application of animal cloning by somatic cell nuclear transfer (SCNT) has been hampered by extremely low success rate and survivors frequently display abnormalities, so specific functions of proteins in placenta have been investigated at the molecular levels during pregnancy. Expand
Proteolytic Activity of the 26S Proteasome Is Required for the Meiotic Resumption, Germinal Vesicle Breakdown, and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes Matured In Vitro1
It is concluded that proteasomal proteolysis has multiple functions in the progression of oocyte meiosis beyond GV and metaphase I stage, polar body extrusion, and cumulus expansion. Expand
Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization
The 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization, and the recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for the success of porcine/mammalian fertilization in vitro. Expand