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Early embryonic lethality caused by targeted disruption of the TRAF-interacting protein (TRIP) gene.
TLDR
Results indicate that TRIP is an essential factor during early mouse embryonic development in vivo, and this work has generated TRIP-deficient mice that were found to die shortly after implantation due to proliferation defects and excessive cell death. Expand
Effects of cryopreservation on Ca2+ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa.
TLDR
Cryopreservation reduces the responsiveness of spermatozoa to depolarization, modulators of the internal Ca2+ store and progesterone in terms of the Ca2+. Expand
Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization1
TLDR
It is concluded that sperm-acrosomal UCHs are involved in sperm-ZP interactions and antipolyspermy defense and could facilitate the management of polysPermy during IVF and provide insights into male infertility. Expand
Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer.
TLDR
The production of transgenic pigs using somatic cell nuclear transfer and a technique for removing nontransfected donor cells and for creating different founder animals has not yet been fully elucidated provide a valuable experimental model for applying and evaluating transgenic technology in pigs. Expand
Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductalExpand
Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17째C and 4째C, and Frozen-thawed Semen
TLDR
It was found out that the original quality of boar semen with cold shock resistance sperm played an important role and the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. Expand
46 DIFFERENTIAL PATTERNS OF PROTEIN SYNTHESIS BETWEEN NORMAL AND CLONED PLACENTAE
TLDR
Practical application of animal cloning by somatic cell nuclear transfer (SCNT) has been hampered by extremely low success rate and survivors frequently display abnormalities, so specific functions of proteins in placenta have been investigated at the molecular levels during pregnancy. Expand
Proteolytic Activity of the 26S Proteasome Is Required for the Meiotic Resumption, Germinal Vesicle Breakdown, and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes Matured In Vitro1
TLDR
It is concluded that proteasomal proteolysis has multiple functions in the progression of oocyte meiosis beyond GV and metaphase I stage, polar body extrusion, and cumulus expansion. Expand
Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization
TLDR
The 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization, and the recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for the success of porcine/mammalian fertilization in vitro. Expand
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