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Journals and Conferences
A gene encoding the mature form of human growth hormone (hGH) was fused to the secretion signal coding sequence of the Escherichia coli heat-stable enterotoxin II (STII). This hybrid gene was preceded by two Shine-Dalgarno sequences derived from the trp and STII-coding genes and was expressed in E. coli under the transcriptional control of the E. coli… (More)
Shannon information (SI) and its special case, divergence, are defined for a DNA sequence in terms of probabilities of chemical words in the sequence and are computed for a set of complete genomes highly diverse in length and composition. We find the following: SI (but not divergence) is inversely proportional to sequence length for a random sequence but is… (More)
We have studied the synthesis, secretion, and processing of human growth hormone (hGH) in Escherichia coli transformed with plasmids engineered for the expression of hGH as a secreted product. In one plasmid, pPreHGH207-2, the coding sequence of the natural hGH precursor (pre-hGH) is placed under the control of the E. coli trp promoter. In a second plasmid,… (More)
Shannon information in the genomes of all completely sequenced prokaryotes and eukaryotes are measured in word lengths of two to ten letters. It is found that in a scale-dependent way, the Shannon information in complete genomes are much greater than that in matching random sequences - thousands of times greater in the case of short words. Furthermore, with… (More)
Complete genome sequences Complete sequences of the 155 prokaryotes are taken from GenBank: yeast, 3). Fig. S1 gives an indication of the diversity of the complete sequences in terms of sequence length L and fractional A+T content p among all sequences.
We report the discovery of a set of universal lengths that characterize all microbial complete genomes. The Shannon information [Shannon 1948] of 108 complete microbial genomes relative to those of their respective randomized counterparts are computed and the results are summarized in a two-parameter exponential relation: L r (k) = (42 ± 21) × 2.64 k , 2 ≥… (More)
The abundance of duplications in genomes in the form of paralogs, pseudogenes and a variety of repeats suggests that genomes may have used duplications as one mode for their growth. However a systematic knowledge on all possible duplications in whole genomes is still lacking. This paper reports the results of a detailed study of occurrence frequencies of… (More)
Statistical analysis of distributions of occurrence frequencies of short words in 108 microbial complete genomes reveals the existence of a set of universal " root-sequence lengths " shared by all microbial genomes. These lengths and their universality give powerful clues to the way microbial genomes are grown. We show that the observed genomic properties… (More)
Hog cholera virus antigens were found densely distributed in skin and tongue of pigs experimentally infected with hog cholera virus. The finding described here warrants the usage of ear biopsies for hog cholera diagnosis on a herd basis.