Chad M. Gonzales

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Background: Pah1p is a phosphatidic acid phosphatase that generates diacylglycerol. Results: Deletion of PAH1 alters the sorting of fusion factors to the vacuole and inhibits fusion. Conclusion: Conversion of phosphatidic acid to diacylglycerol is integral to vacuole homeostasis. Significance: This is the first report that links a Lipin1 homologue to the(More)
Russell-Silver syndrome (RSS) is a genetic syndrome with clinical manifestations of intrauterine and postnatal growth retardation, normal head circumference, body asymmetry, and distinctive facial appearance. We followed an infant diagnosed with RSS who had occurrence of multiple gastrointestinal complications. Although there are a number of published(More)
The 14,955-bp Dictyostelium discoideum nuclear plasmid Ddp5 contains six transcribed open reading frames. One of these is related to the rep gene of the Ddp2 plasmid, and the other five are related to genes present on the Ddp1 plasmid. The absence of a homolog of the Ddp1 G1 gene, coupled with the presence of the Ddp2 rep gene homolog and of a 1.6-kb(More)
Since the Human Immunodeficiency Virus was discovered in 1983, there have been about 60 million people infected worldwide, and over 4 million children have been infected under the age of 15. In 2003, there were about 5 million newly infected individuals, of which 700,000 were children under the age of 15. There are an estimated 40,000 new HIV infections per(More)
Dictyostelium plasmids Dgp1 and Dfp1, two members of the Ddp2 plasmid family, are 86% identical in nucleotide sequence. These small (4481 and 5015 bp), high copy number, nuclear plasmids carry both a gene homologous to the Ddp2 rep gene and a long 0.47- to 0. 48-kb inverted repeat region. Their Rep proteins are 82.8% identical in amino acid sequence and(More)
Ddp6 is a high-copy number, circular plasmid found in the nucleus of the simple eukaryote Dictyostelium discoideum wild isolate NC47.2. The complete nucleotide sequence, 5257 bp, shows that Ddp6 has a structure similar to that of other members of the Ddp2 plasmid family: a single long 2.8-kb open reading frame (rep gene) and an inverted repeat containing a(More)
The cytostatic activity of several triterpenequinone methides isolated from Rzedowskia tolantonguensis and Maytenus horrida (Celastraceae) was evaluated against HeLa cell cultures. Their ID50 were determined and compared with those of other related compounds. A preliminary study of the antibacterial activity of the above triterpenequinone methides was also(More)
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