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Using a newly developed platinum-O2-microeletrode [30] based on the design ofSilver [37] the construction and properties of which are described,pO2-measurements in the parenchyma of the blood-perfused and the cell-free perfused rat kidney were carried out. By continuous recording of thepO2 during slow (150 μ×min−1) insertion of the O2-electrode into the(More)
In mongrel dogs renal venous blood flow was continuously measured by means of an electromagnetic flowmeter, and the pressure in the renal artery was monitored by a Statham transducer. Short lasting diastolic elevations of arterial pressure (pressure steps) were induced by arterial counterpulsation (diastolic augmentation), i.e. rapid pumping of a variable(More)
BACKGROUND The contact system is generally believed to be the main trigger of the coagulation cascade during extracorporeal circulation. However, the extent of contact activation, its role for intradialytic thrombin generation as well as the influence of different dialyzer membranes have not been well established. METHODS In a novel full-scale ex vivo(More)
It is generally assumed that the O2 supply to the kidneys is the major determinant of the synthesis of erythropoietin (Ep). In the present study, the O2 supply of the kidneys of rats was lowered by the reduction of renal blood flow (rbf). Plasma Ep was determined after about 18 h of bilateral application of Goldblatt clips with graded inner diameters. The(More)
Several constituents of chromaffin granules were quantitatively determined in noradrenaline and adrenaline cells purified from bovine adrenal medulla. As far as secretory peptides are concerned noradrenaline granules contained slightly more secretogranin II, but much less chromogranin A than adrenaline granules. This can be explained by the dependence of(More)
AIM To assess the usefulness of ultrasound in the diagnosis of rupture of the distal biceps brachii muscle by demonstrating the clear differentiation between the distal biceps muscle and the surrounding soft tissue in a standardized section of the elbow. METHOD Under sonographic guidance five biceps muscles of three cadavers were marked with a needle in(More)
Two experimental approaches were used to study the processing of chromogranin B and secretogranin II by prohormone convertases. In GH3 cells various prohormone convertases were overexpressed together with the substrate chromogranin B by use of a vaccinia virus infection system. PC1 appeared to be by far the most active enzyme and converted chromogranin B to(More)
The protein NESP55, a new member of the chromogranin family, is present in large dense-core secretory granules of neuroendocrine tissues. We investigated its cellular distribution in adrenal medulla with immunohistochemistry and in situ hybridization. A preferential co-localization of NESP55 with phenylethanolamine-N-methyltransferase in the adrenergic cell(More)
Polypeptide growth factors secreted from the target tissue determine the choice of transmitter synthesis in the innervating nerves. We have investigated whether they also influence the expression of chromogranins and neuropeptide Y, components co-stored with the neurotransmitters within large dense-core vesicles. IMR-32 and SH-SY5Y human neuroblastoma cells(More)