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An encapsulation device, designed on the basis of the laminar jet break-up technique, is characterized for cell immobilization with different types of alginate. The principle of operation of the completely sterilizable encapsulator, together with techniques for the continuous production of beads from 250 microm to 1 mm in diameter, with a size distribution(More)
PRIMARY OBJECTIVE Microencapsulation is a novel method for in vivo immunoprotection of genetically engineered mammalian cells. This study aimed at optimizing alginate/poly-l-lysine/alginate (APA) microencapsulation of mammalian cells in small size (< 300 microm) hollow core microcapsules and at evaluating some of the physical characteristics of APA(More)
Alginate-polylysine-alginate (APA) microcapsules are of particular interest for their application as implants or for bioreactor cultures. Although their formation has been widely studied, there is still a lack of quantitative data describing resistance, membrane thickness and permeability. In this study, the quantitative application of a Texture Analyser(More)
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