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Temperature- and flow-enhanced detection specificity of mutated DNA against the wild type with reporter microspheres.
TLDR
It was shown that at room temperature, flow initially increased the binding of both the MT and WT at lower flow rates but decreased the binding at flow rates ≥4 ml min(-1) due to the increase in the flow-induced impingement force on the FRMs to overcome the bound pDNA and the WT to the GCG at higher flow rates.
DNA hybridization detection with 100 zM sensitivity using piezoelectric plate sensors with an improved noise-reduction algorithm.
TLDR
Real-time, in situ hybridization detection of target DNA (tDNA) in a buffer solution and in urine using 8 μm-thick lead magnesium niobate-lead titanate (PMN-PT) piezoelectric plate sensors (PEPSs) and a new multiple-parabola (>50) resonance peak position fitting algorithm is examined.
Specific in situ hepatitis B viral double mutation (HBVDM) detection in urine with 60 copies ml(-1) analytical sensitivity in a background of 250-fold wild type without DNA isolation and
TLDR
In situ detection of hepatitis B virus 1762T/1764A double mutation (HBVDM) in urine using a (PMN-PT) piezoelectric plate sensor coated with a 16-nucleotide (nt) probe DNA (pDNA) complementary to the HBVDM is examined.
Amplification-free in situ KRAS point mutation detection at 60 copies per mL in urine in a background of 1000-fold wild type.
TLDR
Under such conditions, PEPS was shown to specifically detect KRAS MT in situ with 60 copies per mL analytical sensitivity in a background of clinically-relevant 1000-fold more WT in 30 min without DNA isolation, amplification, or labeling.
Piezoelectric Plate Sensor (PEPS) for Analysis of Specific KRAS Point Mutations at Low Copy Number in Urine Without DNA Isolation or Amplification.
TLDR
Under these conditions PEPS was shown to specifically detect KRAS MT in situ within 30 min with an analytical sensitivity of 60 copies/mL in a clinically relevant background of WT with concentrations 1000-fold greater than that of MT without DNA isolation, amplification, or labeling.
In situ detection of transrenal gene mutations without DNA isolation and amplification using Array Piezoelectric Plate Sensor (PEPS)
TLDR
PEPS was developed as a tool for rapid, label-free, multiplexed and highly sensitive detection of transrenal gene mutations of distant organs in urine and exhibited unprecedented Molar sensitivity in labelfree detection of single-stranded DNA.
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