Learn More
1. Ciliary activity is significantly influenced by chemical and physical properties of the liquid medium in which the cilia beat. 2. We studied the effect of changes in pH, ionic strength and viscosity on the ciliary beat frequency (CBF) of explants of human respiratory mucosa. 3. Optimal CBF was elicited at pH 7 . 0-9 . 0, with a marked reduction of CBF(More)
BACKGROUND A combination of molecular cytogenetic and expression array analysis has been performed on head and neck squamous cell carcinoma (HNSCC) of the oral cavity and supraglottis. These studies were performed to identify consensus regions of chromosomal imbalance and structural rearrangement to determine whether genes located in these genomic regions(More)
Synthetic genes encoding the 190 amino acid Trichoderma reesei xylanase II (TrX) and the closely related Trichoderma viride xylanases have been synthesized in a two-step procedure. Initially, a partial gene encoding amino acids 92-190 was constructed in fusion with the N-terminal half of the Bacillus circulans xylanase (BcX). The remaining BcX gene sequence(More)
The effects of external nutrients on the growth and radiation response of EMT6/Ro spheroids were studied by maintaining spheroids in media with different concentrations of glucose, amino acids, and vitamins. Compared to spheroids grown in normal glucose concentration (5.5 mM), spheroids grown in higher glucose media (24.8 mM), demonstrated no difference in(More)
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of(More)
OBJECTIVE To describe the use of propranolol as first-line treatment or as single therapy to control the proliferating phase of infantile haemangioma in Chinese children. DESIGN Retrospective study. SETTING Regional hospital, Hong Kong. PATIENTS Children 3 years old or younger with facial haemangioma who took oral propranolol between 1 December 2008(More)
Overreplication of DNA associated with gene amplification and drug resistance has been reported to occur after transient hypoxia of rodent cells. Because oxygen levels fluctuate in solid tumors, clinical drug resistance might be stimulated by this mechanism. We have therefore studied the effect of transient hypoxia on sensitivity to doxorubicin in human and(More)
The correlation between intracellular accumulation of doxorubicin (DOX) and drug cytotoxicity was studied in cells from seven cell lines of rodent and human origin. We used three Chinese hamster ovary cell lines (AuxB1, CHRC5, and UV20), two murine tumor cell lines (KHT-LP1 and EMT6/Ro), and two human tumor cell lines (MGH-U1 and DLD-1). Intracellular DOX(More)
An efficient expression system for a low-molecular mass xylanase in Escherichia coli has been developed. A gene encoding the mature Bacillus circulans (Bc) xylanase was designed to imitate the frequency of degenerate codons used in E. coli. Appropriate degenerate codons were used to create multiple unique restriction sites for future mutagenesis studies.(More)
Twenty-eight sputum samples collected from 20 patients with chronic bronchial asthma of atopic and intrinsic clinical types were incubated with human bronchial explants to study their influence on ciliary motility. Of these, 19 (68%) of the sputa exerted a ciliary inhibitory effect of varying degree in a two-hour period. Analysis of the data indicates that(More)