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Structure and Mechanism of a Metal-Sensing Regulatory RNA
Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metalExpand
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Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.
The M-box riboswitch couples intracellular magnesium levels to expression of bacterial metal transport genes. Structural analyses on other riboswitch RNA classes, which typically respond to a smallExpand
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Menaquinone biosynthesis potentiates haem toxicity in Staphylococcus aureus
Staphylococcus aureus is a pathogen that infects multiple anatomical sites leading to a diverse array of diseases. Although vertebrates can restrict the growth of invading pathogens by sequesteringExpand
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Assessment of the requirements for magnesium transporters in Bacillus subtilis.
Magnesium is the most abundant divalent metal in cells and is required for many structural and enzymatic functions. For bacteria, at least three families of proteins function as magnesiumExpand
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The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction
Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we herein visualize protein and metal distributions within PseudomonasExpand
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Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension.
RNA sequences fold upon themselves to form complex structures. Functional analysis of most biological RNAs requires knowledge of secondary structure arrangements and tertiary base interactions.Expand
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Real-time molecular beacon NASBA reveals hblC expression from Bacillus spp. in milk.
Nucleic acid sequence-based amplification (NASBA) was applied in combination with a fluorescein-conjugated molecular beacon specific for a sequence flanked by transcript-specific primers in order toExpand
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Differential activation of Staphylococcus aureus heme detoxification machinery by heme analogues.
The reactive nature of heme enables its use as an enzymatic cofactor while rendering excess heme toxic. The importance of heme detoxification machinery is highlighted by the presence of various typesExpand
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Identification of bacteria causing acute otitis media using Raman microspectroscopy
Otitis media (OM) is the leading cause of acute physician visits and prescription of antibiotics for children. Current standard techniques to diagnose acute otitis media (AOM) are limited by theirExpand
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Structural features of metabolite-sensing riboswitches.
Riboswitches, metabolite-sensing RNA elements that are present in untranslated regions of the transcripts that they regulate, possess extensive tertiary structure to couple metabolite binding toExpand
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