Catharina de L Davies

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The mechanism involved in the ultrasound-enhanced intracellular delivery of fluorescein-isothiocyanate (FITC)-dextran (molecular weight 4 to 2000 kDa) and liposomes containing doxorubicin (Dox) was studied using HeLa cells and an ultrasound transducer at 300 kHz, varying the acoustic power. The cellular uptake and cell viability were measured using flow(More)
Focused ultrasound (FUS) in the presence of microbubbles transiently and reversibly opens the blood-brain barrier (BBB) in rodents and humans, thereby providing a time window for increased drug delivery into brain tissue. To get insight into the underlying mechanisms that govern ultrasound (US)-mediated opening of the BBB, in vitro models are a useful(More)
BACKGROUND Multicell spheroids from the human osteosarcoma cell line, OHS, were incubated with increasing concentrations of the monoclonal antibodies TP-1, TP-3 and 9.2.27 having different affinities (Ka = 8.5 x 10(8) M-1, 3.4 x 10(9) M-1 and 1.4 x 10(11) M-1, respectively). MATERIALS AND METHODS Uptake and penetration of the fluorescein labelled(More)
Penetration and binding of monoclonal antibody (MAb) in multicell osteosarcoma spheroids have been studied by autoradiography and confocal laser scanning microscopy (CLSM). Optical sectioning of the 3-dimensional spheroids was performed by CLSM. Owing to attenuation of fluorescence intensity, FITC-labelled MAb could not be detected at depths greater than 60(More)
Combining ultrasound exposure and liposomal encapsulated anti-cancer drugs has beneficial synergistic effects in cancer therapy, although little is known about the underlying mechanisms. This study has focused on investigating the effect of different ultrasound exposures (1 MHz and 300 kHz) on delivery and distribution of liposomal doxorubicin in Balb/c(More)
Focused ultrasound (FUS) in the presence of microbubbles transiently and reversibly opens the blood-brain barrier (BBB) in rodents and humans, thereby providing a time window for increased drug delivery into brain tissue. To get insight into the underlying mechanisms that govern ultrasound (US)-mediated opening of the BBB, in vitro models are a useful(More)
Ultrasound enhances the release of drugs from liposomes, and in solution this is shown to be caused by cavitation. However, the mechanism in tissue is unclear, and there is a need to bridge the gap between results obtained in solution and in tissue. Thus, we studied the release of liposomal doxorubicin in 5% w/v type I rattail collagen gels when subjected(More)
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