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Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity*
Background: The CORVET and HOPS complexes regulate endosomal cargo trafficking but have not been well characterized in mammals. Results: A detailed analysis of subunit interactions within theExpand
Vps33B is required for delivery of endocytosed cargo to lysosomes
Lysosomes are the main degradative compartments of eukaryotic cells. The CORVET and HOPS tethering complexes are well known for their role in membrane fusion in the yeast endocytic pathway. YeastExpand
Clathrin and clathrin adaptor AP-1 control apical trafficking of megalin in the biosynthetic and recycling routes
Megalin (gp330, LRP-2) is a protein structurally related to the low-density lipoprotein receptor family that displays a large luminal domain with multiligand binding properties. Megalin localizes toExpand
CORVET, CHEVI and HOPS – multisubunit tethers of the endo-lysosomal system in health and disease
ABSTRACT Multisubunit tethering complexes (MTCs) are multitasking hubs that form a link between membrane fusion, organelle motility and signaling. CORVET, CHEVI and HOPS are MTCs of theExpand
An adapted protocol to overcome endosomal damage caused by polyethylenimine (PEI) mediated transfections
Transfection of cells using polyethylenimine (PEI) polymers is a widely used technique for gene insertion and exogenous protein expression that offers several advantages over virus or lipid-basedExpand
Accurate measurement of fast endocytic recycling kinetics in real time
ABSTRACT The fast turnover of membrane components through endocytosis and recycling allows precise control of the composition of the plasma membrane. Endocytic recycling can be rapid, with someExpand