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Packed column sub- and supercritical fluid chromatography (SFC) is a versatile separation method: on the one hand the number of parameters acting on the quality of a separation is very large, and the effects of these parameters can be complex (and not always fully understood). But on another hand, due to numerous advantageous properties of CO2-based mobile(More)
Supercritical fluid chromatography (SFC) has recently benefited of new instrumentation, together with the availability of many ultra-high performance columns (sub -2μm fully porous particles or sub -3μm superficially porous particles), rendering it more attractive than ever. Most of these columns commonly used in SFC were initially developed for HPLC use,(More)
The main tests developed in last 20 years to investigate the chromatographic behaviour and the stationary phase properties are described in this paper. These properties are the hydrophobicity, depending on the surface area and the bonding density, the number of accessible residual silanol groups having sometimes different acidity, which can interact with(More)
Varied types of alkylsiloxane-bonded and fluoroalkylsiloxane-bonded stationary phases, all commercially available, were investigated with subcritical fluid mobile phase. The effect of the alkyl chain length (from C4 to C18) and of the nature of the bonding (fluorodecylsiloxane, phenyl-C18 and polar-embedded-C18) on the chromatographic behaviour was(More)
In this third paper, varied types of polar stationary phases, namely silica gel (SI), cyano (CN)- and amino-propyl (NH2)-bonded silica, propanediol-bonded silica (DIOL), poly(ethylene glycol) (PEG) and poly(vinyl alcohol) (PVA), were investigated in subcritical fluid mobile phase. This study was performed to provide a greater knowledge of the properties of(More)
Event-related functional magnetic resonance imaging was used to investigate the localization of syntactic processing in sentence comprehension. Matched pairs of sentences containing identical lexical items were compared. One member of the pair consisted of a syntactically simpler sentence, containing a subject relativized clause. The second member of the(More)
This tutorial provides an overview of the possibilities, limitations and analytical conditions of modern analytical supercritical fluid chromatography (SFC) using columns packed with sub-2 μm particles. In particular, it gives a detailed overview of commercially available modern SFC instrumentation and the detectors that can be employed (UV, MS, ELSD, FID,(More)
The use of supercritical fluids as chromatographic mobile phases allows to obtain rapid separations with high efficiency on packed columns, which could favour the replacement of numerous HPLC methods by supercritical fluid chromatography (SFC) ones. Moreover, despite some unexpected chromatographic behaviours, general retention rules are now well(More)
The interest of pharmaceutical companies for complementary high-performance chromatographic tools to assess a product's purity or enhance this purity is on the rise. The high-throughput capability and economic benefits of supercritical fluid chromatography, but also the "green" aspect of CO2 as the principal solvent, render supercritical fluid(More)
In supercritical fluid chromatography (SFC), the effects of adding a co-solvent to carbon dioxide are numerous. In this work, the changes in retention and selectivity occurring when the nature of the co-solvent (methanol, ethanol, isopropanol, acetonitrile) in the SFC mobile phase is varied, are studied on seven columns with different stationary phase(More)