Carol N Nottenburg

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CD44 is a glycoprotein that participates in adhesion of lymphocytes to high endothelial cells of lymph organs and likely functions in other intercellular adhesions as well. We have isolated a mouse CD44 cDNA by polymerase chain reaction amplification of cDNA synthesized from total cellular RNA isolated from 38C-13, a B-lymphocyte cell line. The(More)
The surface phenotype of Peyer's patch germinal center lymphoid cells in the mouse is described. It is confirmed that most germinal center lymphocytes bind high levels of peanut agglutination (PNA), a lectin with specificity for terminal galactosyl residues. It is shown that germinal center lymphocytes can be identified in cell suspensions as a discrete(More)
The Rockefeller Foundation, 420 Fifth Avenue, New York, New York 10018–2702 (D.P.D.); Center for the Application of Molecular Biology to International Agriculture (CAMBIA), G.P.O. Box 3200, Canberra, Australian Capital Territory 2601, Australia (C.N.); Bio Economic Research Associates, LLC, 1711 Franklin Street, Berkeley, California 94702–1412 (G.D.G.); and(More)
“As a horticulturalist I protest the movement and hope Congress pays no attention to the matter. The originators of valuable varieties have it in their power now to secure ample compensation: and it is their own fault if they do not....In my opinion, the influence of a patent law of this kind would be to retard the dissemination of new things and in this(More)
The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by high-walled endothelial cells. Lymphocyte adhesion to and extravasation at these sites is associated with the expression of specific lymphoid(More)
Lymphocyte adhesion to high endothelial venule cells in lymphoid organs of mice is mediated by several cell-surface glycoproteins, one of which, gp90MEL-14, is detected by the MEL-14 monoclonal antibody (mAb). The MEL-14 mAb was used to select two variants of the EL4 cell line, EL4MEL-14-hi and EL4-MEL-14-lo, that have disparate cell surface expression of(More)
From a library of mouse sperm DNA, we have isolated two overlapping clones which contain the C(delta) gene. One of these clones also contains the C(mu) gene. The C(delta) gene is separated from the C(mu) membrane exons by approximately 2 kilobases (kb) of DAN. The C(delta) gene was identified by (a) hybridization to poly(A)(+)RNA prepared from the(More)
The heavy chain variable region composition of the human adult Ab repertoire is poorly defined, but recent evidence suggests that peripheral blood B cells may express a nonstochastic assortment of VH genes. In this study, the contribution of individual VH gene segments to the human Ab repertoire has been assessed. As a measure of VH gene utilization, the(More)
Allelic exclusion of immunoglobulin gene products results in the expression of only one of two possible alleles in normal B lineage cells. Attempts to define the role of heavy chain gene rearrangements in this process have revealed the nonexpressed allele to be rarely in its germline context, but rather incompletely rearranged (D/J rearrangements) or(More)
The antibody response to Haemophilus influenzae type b polysaccharide (Hib PS) is known to be encoded by a few V-region genes. We have obtained four human monoclonal Hib PS antibodies from four healthy adult subjects immunized with diphtheria toxin-conjugated Hib PS vaccine. The VH gene segments that encode for these antibodies belong to the VH3 gene(More)