Carol J. Maslansky

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Hepatocyte primary cultures (HPC) derived from rat, mouse, hamster, and rabbit liver were characterized for a variety of parameters. The conditions that maximized recovery, attachment, and survival varied between species. Hepatocytes from all four species were capable of attaching in serum-free Williams’ medium E (WME), but optimal attachment as monolayer(More)
Genotoxicity was not elicited by any of a variety of organochlorine pesticides tested in the hepatocyte primary culture (HPC)/DNA repair assay utilizing hepatocytes from the rat, mouse, and hamster. DNA repair was observed in response to the positive control in all three systems. Thus the tumorigenicity of the organochlorine pesticides appears to reflect an(More)
The relationship between acetylation rates of rabbit hepatocytes and their susceptibility to genotoxicity by DNA-damaging chemicals that undergo N-acetylation was studied in primary cultures of hepatocytes from New Zealand White rabbits that have a genetically determined difference in acetylation rates. Hepatocytes from rapid and slow acetylator rabbits(More)
Continuous epithelial-like cell lines derived from normal adult rat liver and hepatocarcinomas were evaluated for their growth in soft agar and five properties of the cell membrane as markers for neoplastic transformation. A correlation of these properties was made to the tumorigenicity of the lines in nude mice. Growth in soft agar was a specific and(More)
An extensive survey of markers for transformation of adult rat liver-derived epithelial-like cell lines was conducted. The cell surface properties associated with tumorigenicity were reported earlier. Now we report the correlation of various growth characteristics and enzyme activities with tumorigenic ity. Among markers identified that correlated with(More)
The DNA repair capabilities of cultured hepatocytes derived from five mammalian species in response to a broad range of ultraviolet light exposures were measured. Differences in the induction of DNA repair synthesis and the proportion of responding cells were noted only for the lowest fluences. These differences appeared to be positively correlated with the(More)
In humans and rabbits, differences in the rate of N-acetylation of aromatic amines are under polymorphic genetic control. Individuals are classified as either rapid or slow acetylators. In the current study, the relationship between acetylator phenotype and susceptibility to the genotoxicities of benzidine, 4-aminobiphenyl,(More)
An extensive survey of markers for transformation of adult rat liver-derived epithelial-like cell lines was conducted. The cell surface properties associated with tumorigenicity were reported earlier. Now we report the correlation of various growth characteristics and enzyme activities with tumorigenic ity. Among markers identified that correlated with(More)
The intestinal carcinogen 3,2'-dimethyl-4-aminobiphenyl (DMAB) and the hepatocarcinogen N-2-acetylaminofluorene (AAF) were administered intraperitoneally to male F344 rats to determine if both would produce DNA damage in the liver. Hepatocyte primary cultures (HPC) were prepared and DNA-repair was assessed in vitro by autoradiography. Hepatocytes from both(More)