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The size and complexity of conifer genomes has, until now, prevented full genome sequencing and assembly. The large research community and economic importance of loblolly pine, Pinus taeda L., made it an early candidate for reference sequence determination. We develop a novel strategy to sequence the genome of loblolly pine that combines unique aspects of(More)
BACKGROUND In today's age of genomic discovery, no attempt has been made to comprehensively sequence a gymnosperm genome. The largest genus in the coniferous family Pinaceae is Pinus, whose 110-120 species have extremely large genomes (c. 20-40 Gb, 2N = 24). The size and complexity of these genomes have prompted much speculation as to the feasibility of(More)
The largest genus in the conifer family Pinaceae is Pinus, with over 100 species. The size and complexity of their genomes (∼20-40 Gb, 2n = 24) have delayed the arrival of a well-annotated reference sequence. In this study, we present the annotation of the first whole-genome shotgun assembly of loblolly pine (Pinus taeda L.), which comprises 20.1 Gb of(More)
Loblolly pine (Pinus taeda L.) is one of the most widely planted and commercially important forest tree species in the USA and worldwide, and is an object of intense genomic research. However, whole genome resequencing in loblolly pine is hampered by its large size and complexity and a lack of a good reference. As a valid and more feasible alternative,(More)
Millettia pinnata (formerly Pongamia pinnata) is a fast-growing leguminous tree indigenous to the Indian subcontinent, Southeast Asia, and Australia. This species has been introduced to subtropical and arid regions of Africa, India, the Philippines, Malaysia, Australia, and the USA for commercial growth. Exhibiting saline and drought tolerance, as well as(More)
Sugar pine (Pinus lambertiana Douglas) is within the subgenus Strobus with an estimated genome size of 31 Gbp. Transcriptomic resources are of particular interest in conifers due to the challenges presented in their megagenomes for gene identification. In this study, we present the first comprehensive survey of the P. lambertiana transcriptome through deep(More)
Erratum The accession number for the sequence submitted to NCBI Sequence Read Archive (SRA) listed in the original article [1] is incorrect; SRP075763 should be SRP075363. Reference 1. Lu M, et al. Exome genotyping, linkage disequilibrium and population structure in loblolly pine (Pinus taeda L. permits unrestricted use, distribution, and reproduction in(More)
The largest genus in the conifer family Pinaceae is Pinus, with over 100 species. The size and complexity of their genomes (20–40 Gb, 2n = 24) have delayed the arrival of a well-annotated reference sequence. In this study, we present the annotation of the first whole-genome shotgun assembly of loblolly pine (Pinus taeda L.), which comprises 20.1 Gb of(More)
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