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Epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral(More)
In this study, we have analyzed the ontogenic expression of three gonadotrophin-releasing hormones (GnRH) systems expressed in the brain of a perciform fish, the European sea bass, using in situ hybridization. The riboprobes used correspond to the GnRH-associated peptide (GAP) coding regions of the three prepro-GnRH cDNAs cloned from the same species:(More)
We report the distribution of serotonin immunoreactive (5-HT-ir) structures in the brain of the adult Senegalese sole, Solea senegalensis, using the streptavidin-biotin-peroxidase complex immunohistochemical method. We have found a wide distribution of immunoreactive fibers throughout the entire brain. 5-HT-ir cell bodies appeared restricted to some(More)
The localization of catecholamines in the brain of the Senegalese sole was determined by immunohistochemical techniques using antibodies against tyrosine hydroxylase. Although the general pattern of distribution of catecholamines is consistent with that reported in other teleosts, some remarkable differences are observed. The most rostral tyrosine(More)
The Senegalese sole (Solea senegalensis) is a valuable flatfish for aquaculture, but it presents important reproductive problems in captivity. Spawning is achieved by wild-caught breeders but cultured broodstocks fail to spawn spontaneously and, when they do, eggs are unfertilized. To gain knowledge on the physiological basis underlying this reproductive(More)
The gonadotropins (GTHs), follicle stimulating hormone (FSH) and luteinizing hormone (LH), determine the reproductive competence of adult breeders, but also participate in the establishment of the reproductive axis at early stages of life. The present study aimed at studying, by real-time qPCR, the gene expression levels of GTH subunits (FSHbeta, LHbeta and(More)
The Batrachoididae family is a group of marine teleosts that includes several species with more complicated physiological characteristics, such as their excretory, reproductive, cardiovascular and respiratory systems. Previous studies of the 5S rDNA gene family carried out in four species from the Western Atlantic showed two types of this gene in two(More)
We present the results of an immunohistochemical study aimed at localizing the neuropeptide Y (NPY) in the brain of the Senegalese sole, Solea senegalensis, using an antiserum raised against porcine NPY and the streptavidin-biotin-peroxidase method. In this species, we have identified immunoreactive cells in the ventral and dorsal telencephalon, caudal(More)
An histological and histochemical study was conducted on the stomach of adult Senegal sole, Solea senegalensis specimens. The stomach was made up of four distinct layers: mucosa, lamina propria-submucosa-, muscularis and serosa. Surface epithelial, glandular and rodlet cells were present in the mucosa. Cells of the columnar epithelium contained a basal(More)
The present paper deals with the ontogeny of the forebrain of the flatfish Senegal sole, Solea senegalensis, through different developmental stages before and after to metamorphosis. A first approach was made by conventional histological techniques, which allowed the determination of the main ontogenetic events. A second approach was to analyze the(More)