Carmen Sánchez-Soto

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Pancreatic islets are not fully developed at birth and it is not clear how they are vascularised and innervated. Nerve Growth Factor (NGF) is required to guide sympathetic neurons that innervate peripheral organs and also in cardiovascular system and ovary angiogenesis. Pancreatic beta cells of a transgenic mouse that over-expressed NGF in attracts(More)
Function and structure of adult pancreatic islets are determined by early postnatal development, which in rats corresponds to the first month of life. We analyzed changes in blood glucose and hormones during this stage and their association with morphological and functional changes of alpha and beta cell populations during this period. At day 20 (d20),(More)
Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights(More)
WILLY J. MALAISSE,1 LEONARD C. BEST,2 ANDRÉ HERCHUELZ,3 MARCIA HIRIART,4 HASSAN JIJAKLI,1 MARCEL M. KADIATA,1 ELENA LARRIETA-CARASCO,4 AOUATIF LAGHMICH,1 KARIM LOUCHAMI,1 DANY MERCAN,1 ELIZABETH OLIVARES,1 CARMEN SÀNCHEZ-SOTO,4 OLIVIER SCRUEL,1 ABDULLAH SENER,1 ISABEL VALVERDE,5 MARÍA L. VILLANUEVA-PEÑACARRILLO,5 CONCEPCIÓN VIÑAMBRES,5 AND WALTER S.(More)
WILLY J. MALAISSE, CARMEN SÁNCHEZ-SOTO, M. ELENA LARRIETA, MARCIA HIRIART, HASSAN JIJAKLI, CONCEPCIÓN VIÑAMBRES, MARÍA L. VILLANUEVA-PEÑACARRILLO, ISABEL VALVERDE, OLE KIRK, MARCEL M. KADIATA, AND ABDULLAH SENER Laboratory of Experimental Medicine, Brussels Free University, B-1070 Brussels, Belgium; Department of Biophysics, Institute of Cellular(More)
The functional determinants of the insulinotropic action of alpha-D-glucose pentaacetate were investigated in rat pancreatic islets. The ester mimicked the effect of nutrient secretagogues by recruiting individual B cells into an active secretory state, stimulating proinsulin biosynthesis, inhibiting 86Rb outflow, and augmenting 45Ca efflux from prelabeled(More)
The metabolism of beta-L-glucose pentaacetate and its interference with the catabolism of L-[U-14C]glutamine, [U-14C]palmitate, D-[U-14C]glucose, and D-[5-3H]glucose were examined in rat pancreatic islets. Likewise, attention was paid to the effects of this ester on the biosynthesis of islet peptides, the release of insulin from incubated or perifused(More)
Transient receptor potential channels, especially the members of the melastatin family (TRPM), participate in insulin secretion. Some of them are substrates for protein kinases, which are involved in several neurotransmitter, incretin and hormonal signaling cascades in β cells. The functional relationships between protein kinases and TRPM channels in(More)
Glucose-induced insulin secretion by pancreatic beta-cells depends on membrane depolarization and [Ca2+]i increase. We correlated voltage- and current-clamp recordings, [Ca2+]i measurements, and insulin reverse hemolytic plaque assay to analyze the activity of a thapsigargin-sensitive cationic channel that can be important for membrane depolarization in(More)
Metabolic syndrome (MS) can be defined as a group of signs that increases the risk of developing type 2 diabetes mellitus (DM2). These signs include obesity, hyperinsulinemia and insulin resistance. We are interested in the mechanisms that trigger hyperinsulinemia as a step to understand how β cells fail in DM2. Pancreatic β cells secrete insulin in(More)