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AIMS To determine the biodiversity of rhizobial strains nodulating Cicer arietinum L. in representative soils from various areas of Morocco. METHODS AND RESULTS Symbiotic traits, utilization of 49 carbohydrate sources, resistance to antibiotics and heavy metals, tolerance to salinity, to extreme temperatures and pH were studied as phenotypic markers. In(More)
In this work the effect of abscisic acid (ABA) and 100 mM NaCl on common bean (Phaseolus vulgaris var. Coco) growth, nitrogenase activity, and nodule metabolism was studied. Experiments were carried out in a controlled environmental chamber and plants, at the vegetative growth stage (16 days old), were treated with ABA (1 lM and 10 lM) and 48 h later were(More)
The effect of NaCl stress (50 mM) and validamycin A treatment (30 mM) on growth and nitrogen fixation of Lotus japonicus was investigated in plants cultured under symbiotic and hydroponics conditions for teen weeks (flowering stage). Validamycin A was used as a potent trehalase inhibitor, and was able to produce a five-fold increase in the level of(More)
Polyamines are cationic molecules that play an important role in the plant response to environmental stresses. The aim of this work is to determine the role of these compounds in the response to salinity of Medicago sativa plants in symbiosis with the soil bacteria Sinorhizobium meliloti. M. sativa plants inoculated with S. meliloti were subjected to 100(More)
Several nitrogen compounds were identified and quantified in the apoplastic and symplastic sap of sugarcane stems. The sap of stems was composed mainly of soluble sugars, which constituted 95% of the total organic compounds detected. Sap also contained nitrogen compounds, with amino acids (50-70% of N) and proteins (20-30% of N), being the main nitrogenous(More)
AIMS To characterize the physiological and metabolic responses of Mesorhizobium ciceri strain ch-191 to salt stress, investigating the changes induced by salinity in protein and lipopolysaccharide profiles, as well as determining the accumulation of amino acids, glutamate and proline. METHODS AND RESULTS Strain ch-191 of M. ciceri was grown with different(More)
The effects of different carbon and nitrogen sources on the growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus were investigated. The amino acids asparagine, aspartic acid, and glutamic acid affected microbial growth and nitrogenase activity. Several enzymatic activities involved in the tricarboxylic acid cycle were(More)
AIMS The effect of some abiotic factors, dryness, heat and salinity on the growth and biological activity of Gluconacetobacter diazotrophicus, and the influence of a salt stress on some enzymes involved in carbon metabolism of these bacteria is studied under laboratory conditions. METHODS AND RESULTS Strain PAL-5 of G. diazotrophicus was incubated under(More)
The purification and characterization of trehalase from common bean nodules as well as the role of this enzyme on growth, nodulation nitrogen fixation by examining the effects of the trehalase inhibitor validamycin A, was studied. Validamycin A did not affect plant and nodule mass, neither root trehalase and nitrogenase activity; however this treatment(More)
The main findings of a cooperative research group of agronomists, plant breeders, microbiologists, physiologists and molecularists to improve the symbiotic nitrogen fixation (SNF) and N2-dependent yield of common bean under moderate salinity in the Mediterranean basin are summarised. Agronomic surveys in reference production areas show large spatial and(More)