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In this work we have investigated the contribution of pretreatment with 0.1 and 0.5mM salicylic acid (SA) to the protection against salt stress in root nodules of Medicago sativa in symbiosis with Sinorhizobium meliloti. SA alleviated the inhibition induced by salinity in the plant growth and photosynthetic capacity of M. sativa-S. meliloti symbiosis. In(More)
AIMS To determine the biodiversity of rhizobial strains nodulating Cicer arietinum L. in representative soils from various areas of Morocco. METHODS AND RESULTS Symbiotic traits, utilization of 49 carbohydrate sources, resistance to antibiotics and heavy metals, tolerance to salinity, to extreme temperatures and pH were studied as phenotypic markers. In(More)
Lotus japonicus and Medicago truncatula model legumes, which form determined and indeterminate nodules, respectively, provide a convenient system to study plant-Rhizobium interaction and to establish differences between the two types of nodules under salt stress conditions. We examined the effects of 25 and 50mM NaCl doses on growth and nitrogen fixation(More)
Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranoside), a non-reducing disaccharide, has been found in a wide variety of organisms playing an important role as an abiotic stress protectant. Plants may come into contact with trehalose from exogenous sources, such as in plant-rhizobia symbiosis in which the rhizobia have the capacity to produce(More)
Characterization of nine transposon-induced mutants of Rhizobium tropici with decreased salt tolerance (DST) allowed the identification of eight gene loci required for adaptation to high external NaCl. Most of the genes also were involved in adaptation to hyperosmotic media and were required to overcome the toxicity of LiCl. According to their possible(More)
The effect of NaCl stress (50 mM) and validamycin A treatment (30 mM) on growth and nitrogen fixation of Lotus japonicus was investigated in plants cultured under symbiotic and hydroponics conditions for teen weeks (flowering stage). Validamycin A was used as a potent trehalase inhibitor, and was able to produce a five-fold increase in the level of(More)
Several nitrogen compounds were identified and quantified in the apoplastic and symplastic sap of sugarcane stems. The sap of stems was composed mainly of soluble sugars, which constituted 95% of the total organic compounds detected. Sap also contained nitrogen compounds, with amino acids (50-70% of N) and proteins (20-30% of N), being the main nitrogenous(More)
In this work the effect of abscisic acid (ABA) and 100 mM NaCl on common bean (Phaseolus vulgaris var. Coco) growth, nitrogenase activity, and nodule metabolism was studied. Experiments were carried out in a controlled environmental chamber and plants, at the vegetative growth stage (16 days old), were treated with ABA (1 lM and 10 lM) and 48 h later were(More)
Polyamines are cationic molecules that play an important role in the plant response to environmental stresses. The aim of this work is to determine the role of these compounds in the response to salinity of Medicago sativa plants in symbiosis with the soil bacteria Sinorhizobium meliloti. M. sativa plants inoculated with S. meliloti were subjected to 100(More)
The effects of different carbon and nitrogen sources on the growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus were investigated. The amino acids asparagine, aspartic acid, and glutamic acid affected microbial growth and nitrogenase activity. Several enzymatic activities involved in the tricarboxylic acid cycle were(More)