Learn More
Fusion proteins made of green fluorescent protein coupled to SNAP-25 or synaptobrevin were overexpressed in bovine chromaffin cells in order to study the role of critical protein domains in exocytosis. Point mutations in the C-terminal domain of SNAP-25 (K201E and L203E) produced a marked inhibition of secretion, whereas single (Q174K, Q53K) and double(More)
Adrenomedullary chromaffin cells express at least two subtypes of acetylcholine nicotinic receptors, which differ in their sensitivity to the snake toxin alpha-bungarotoxin. One subtype is involved in the activation step of the catecholamine secretion process and is not blocked by the toxin. The other is alpha-bungarotoxin-sensitive, and its functional role(More)
The alpha7 subunit is a component of nicotinic acetylcholine receptors expressed in bovine chromaffin cells. The peculiar localization of these receptors at adrenomedullary areas adjacent to the adrenal cortex suggests that factors, probably glucocorticoids, arising from the cortex might diffuse and regulate alpha7 receptor expression. In reporter gene(More)
alpha-Bungarotoxin-sensitive neuronal nicotinic acetylcholine receptors from bovine adrenomedullary chromaffin cells are up-regulated by long-term exposure to phorbol esters. The rise in receptor density is paralleled by an increase in transcripts corresponding to the alpha7 subunit, which is a component of this receptor subtype. Transcriptional activation(More)
  • 1