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A DNA binding and dimerization motif, with apparent amphipathic helices (the HLH motif), has recently been identified in various proteins, including two that bind to immunoglobulin enhancers (E12 and E47). We show here that various HLH proteins can bind as apparent heterodimers to a single DNA motif and also, albeit usually more weakly, as apparent(More)
The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by long stretches of DNA give rise to poly(A)+(More)
We have determined the nucleotide sequence of two transcription units of the AS-C and shown that their potential protein coding regions share two principal domains of homology. Both domains are conserved within the myc protein family, and one of them is highly homologous with the consensus for protein tyrosine kinase substrates. We show in addition that at(More)
The achaete-scute complex (AS-C) and the daughterless (da) genes encode helix-loop-helix proteins which have been shown to interact in vivo and to be required for neurogenesis. We show in vitro that heterodimers of three AS-C products with DA bind DNA strongly, whereas DA homodimers bind weakly and homo or heterocombinations of AS-C products not at all.(More)
We have determined the nucleotide sequence of two genes of the achaete - scute complex (AS-C) and show that they are homologous to two previously sequenced members of the same locus. These four genes are interspersed with other transcription units of unknown function. We also study the expression of one of these genes by in situ hybridization and compare it(More)
Earlier work suggested that the wingless gene of Drosophila is required for cooperation within discrete groups of cells during development. We show that antisense RNA made from a 3 kb transcript produces wingless mutant phenocopies when injected into wild-type eggs, proving that this transcript executes the wingless function. In the accompanying paper,(More)
The expression of a subset of homologous genes of the AS-C is required during embryogenesis and metamorphosis for proper neural development. Here we study the expression of three of these genes (T3, T4, and T5) and show that their transcripts accumulate at the blastoderm stage in periodic patterns coincident with the dorsoventral extent of the(More)
Reactive oxygen species have been implicated as a cause of cancer and aging in mammals. Mice deficient for the antioxidant enzyme CuZn-superoxide dismutase (Sod1) have a decreased life span and an elevated incidence of liver cancer. To test the hypothesis that the cancer-prone phenotype in such mice is due to accelerated spontaneous mutation accumulation,(More)
Complementary DNA clones representing cytoplasmic poly(A) RNAs of sea urchin embryos were hybridized with metabolically labeled cytoplasmic RNA preparations and the rates of appearance and of decay for each transcript species were determined at the blastula-gastrula stage of development. The prevalence of the transcripts chosen for this study ranged, on(More)
The role of bioactive compounds in wound repair is critical. The preliminary work described herein includes the study of the effects of second degree burns in a Rex rabbit model and the action of human umbilical cord cells on the regulation and secretion of bioactive compounds. When applied on blood scaffolds as heterograft matrices, fibroblasts proliferate(More)