Carlos López-Herrera

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In order to monitor Rosellinia necatrix infection of avocado roots, we generated a plasmid vector (pCPXHY1eGFP) constitutively expressing EGFP and developed a protoplast transformation protocol. Using this protocol, four R. necatrix isolates were efficiently transformed and were shown to stably express EGFP homogeneously while not having any observable(More)
UNLABELLED White root rot caused by Rosellinia necatrix is one of the most destructive diseases of many woody plants in the temperate regions of the world, particularly in Europe and Asia. Recent outbreaks of R. necatrix around the globe have increased the interest in this pathogen. Although the ecology of the disease has been poorly studied, recent genetic(More)
The expression of antifungal genes from Trichoderma harzianum, mainly chitinases, has been used to confer plant resistance to fungal diseases. However, the biotechnological potential of glucanase genes from Trichoderma has been scarcely assessed. In this research, transgenic strawberry plants expressing the β-1,3-glucanase gene bgn13.1 from T. harzianum,(More)
Fifty-five isolates of Rosellinia necatrix, the cause of common avocado white root rot disease, were collected from south-east Spain and characterised according to their virulence behaviour and their molecular patterns to assess broader levels of genetic diversity. Virulence properties were revealed by in vitro inoculation on avocado plants. Differences in(More)
This study tested the effectiveness of single and combined applications of Trichoderma and rhizobacterial strains to control white root rot (WRR) caused by Rosellinia necatrix in avocado plants. Three Trichoderma, two T. atroviride and one T. virens monoconidal strains and four bacterial strains (Bacillus subtilis, Pseudomonas pseudoalcaligenes and two P.(More)
To study the relationship between temperature regimes and loss of viability of Dematophora necatrix in soil, two field experiments were conducted to determine the effectiveness of soil solarization on reducing the population of D. necatrix colonizing avocado root segments buried at a depth of 15–60 cm. Increase of maximum hourly temperatures attributable to(More)
By transversely cutting infected avocado plant stems and using PCR techniques on avocado leaves, two experiments were carried out to determine whether Rosellinia necatrix can invade avocado vascular tissues. We were unable to detect the pathogen in either stems or leaves in either experiment, so we concluded that R. necatrix does not invade the vascular(More)
Seven different strains of Trichoderma isolated from avocado roots showed antagonism to Rosellinia necatrix, which is the causal agent of white root rot. We studied these Trichoderma strains on the basis of the secondary metabolites produced in liquid culture. Five different compounds, namely, 6PP (6-pentyl-α-pyrone), Harzianolide(More)
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