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With the capacity for rapid self-renewal and regeneration, the intestinal epithelium is stereotypical of stem cell-supported tissues. Yet the pattern of stem cell turnover remains in question. Applying analytical methods from population dynamics and statistical physics to an inducible genetic labeling system, we showed that clone size distributions conform(More)
In this paper, an alternative procedure for the histochemical detection of HRP using amonium heptamolybdate (AHM) as a stabilizing agent and tetramethyl benzidine (TMB) as a chromogen is reported. This procedure avoids the two main problems that occur in previous methods using sodium nitroferricyanide (SNF) as the stabilizer, namely, the appearance of(More)
Labelled cells were consistently observed in the medial cortex of the lizard brain after i.p. injections of tritiated thymidine (5 microCi/g b. wt.), 1, 7, 18 or 28 days of survival and posterior autoradiographic evaluation. In 3 groups of specimens (postnatal, young and adult) of the species Podarcis hispanica, after one day of survival, labelled cells(More)
Double labelling autoradiography-HRP experiments were performed to examine whether late generated neurons in the medial cortex of lizards develop and send axons to their targets. One to two months after receiving a series of tritiated thymidine ([3H]T) injections to label recently generated neurons, lizards (Podarcis hispanica) were subjected to a HRP(More)
The lizard medial cortex (a zone homologous to the mammalian fascia dentata) shows delayed postnatal neurogenesis throughout the lifetime of these animals. Experimental lesioning of this area is followed by neuronal regeneration, a unique phenomenon in the adult amniote telencephalon. The differential effects of temperature and photoperiod on postnatal(More)
Cells considered to be migratory in the cerebral cortex of adult lizards are ultrastructurally of two types. Nuclei in the first type have highly dispersed chromatin, creating a spongy appearance, whereas in the second type the chromatin is irregularly clumped. Both types of cells are closely associated with processes of radial ependymal glia cells, which(More)
This study reports that lesion of the adult lizard medial cortex (lizard hippocampal fascia dentata) induces a short period of intensive neurogenesis which we have termed reactive neurogenesis; a cell proliferation event that occurs in the subjacent ependyma. Specific lesion of the medial cortex was achieved by intraperitoneal injection of the neurotoxin(More)
Neuronal counts in the medial, dorso-medial, and dorsal areas of the cerebral cortex of a common lizard, Podarcis hispanica, have been performed on a set of different sized (aged) animals. With age, (size), there is a significant increase in the number of neuronal somata of the inner plexiform and granular strata of both the medial and dorso-medial areas.(More)
The GABAergic innervation of the extraocular motor nuclei in the cat was evaluated using postembedding immunocytochemical techniques. The characterization of GABA-immunoreactive terminals in the oculomotor nucleus was carried out at the light and electron microscopic levels. GABA-immunopositive puncta suggestive of boutons were abundant in semithin sections(More)
Young, adult and presumed old specimens of the tropical lizard Tropidurus hispidus, living in an almost steady warm habitat, have been the subjects of a 5-bromodeoxiuridine immunocytochemical study to label proliferating brain cells. All animals showed abundant 5-bromodeoxiuridine-labeled nuclei in the ependyma of their telencephalic lateral ventricles,(More)