Carl B Abulencia

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Low-biomass samples from nitrate and heavy metal contaminated soils yield DNA amounts that have limited use for direct, native analysis and screening. Multiple displacement amplification (MDA) using phi29 DNA polymerase was used to amplify whole genomes from environmental, contaminated, subsurface sediments. By first amplifying the genomic DNA (gDNA),(More)
Current metagenomic approaches to the study of complex microbial consortia provide a glimpse into the community metabolism and occasionally allow genomic assemblies for the most abundant organisms. However, little information is gained for the members of the community present at low frequencies, especially those representing yet-uncultured taxa, which(More)
Carl Abulencia, Eric J. Alm, Gary Anderson, Edward Baidoo, Peter Benke, Sharon Borglin, Eoin L. Brodie, Romy Chakraborty, Swapnil Chhabra, Gabriela Chirica, Dylan Chivian, Michael J. Cipriano, M.E. Clark, Paramvir S. Dehal, Elliot C. Drury, Inna Dubchak, Dwayne A. Elias, Matthew W. Fields, J. Gabster, Sara P. Gaucher, Jil Geller, B. Giles, Masood Hadi,(More)
We are studying relatively simple, low diversity microbial communities associated with extremely acidic, metal-rich mine drainage system to develop an understanding of adaptation, evolution, and the linkages between microbial activity and environmental geochemistry. In order to assess the genetic potential of an entire natural biofilm sample we partially(More)
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