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Validation of reference genes for reverse transcription real‐time quantitative PCR analysis in the deep‐sea bacterium Shewanella psychrophila WP2
- Shunzhang Liu, Canxing Meng, Guanpeng Xu, Huahua Jian, F. Wang
- Biology, Medicine
- FEMS microbiology letters
- 1 April 2018
&NA; Reference genes are critical to obtain reliable results of reverse transcription real‐time quantitative PCR (RT‐qPCR), which is widely used for relative quantification of gene expression. In… Expand
Phylogenomic analysis reveals a two-stage process of the evolutionary transition of Shewanella from the upper ocean to the hadal zone.
Shewanella strains are characterized by versatile metabolic capabilities, resulting in their wide distribution in the ocean at different depths. Considering that particle sedimentation is an… Expand
The thermo-regulated genetic switch of deep-sea filamentous phage SW1 and its distribution in Pacific Ocean.
Viruses, especially bacteriophages are thought to have important functions in the deep-sea ecosystem, but little is known about the induction mechanism of benthic phages towards environmental change.… Expand