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Previously we have shown that nonsyndromic cleft lip with or without cleft palate (NSCL/P) is strongly associated with SNPs in IRF6 (interferon regulatory factor 6). Here, we use multispecies sequence comparisons to identify a common SNP (rs642961, G>A) in a newly identified IRF6 enhancer. The A allele is significantly overtransmitted (P = 1 x 10(-11)) in(More)
Comparative genomic sequence analysis MultiPipMaker, Electrophoretic mobility shift assay (EMSA) Infrared dye (IRDye-700) end-labeled and unlabeled oligonucleotide probes were purchased from LI-COR Biosciences (Lincoln, NE) and IDT (Coralville, IA), respectively. We performed EMSAs by using the LI-COR EMSA Kit. Briefly, 6μg of human recombinant AP-2α(More)
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