Calvin B. Coulter

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1. The movement of normal and sensitized red blood cells in the electric field is a function of the hydrogen ion concentration. The isoelectric point, at which no movement occurs, corresponds with pH 4.6. 2. On the alkaline side of the isoelectric point the charge carried is negative and increases with the alkalinity. On the acid side the charge is positive(More)
The pigment contained in the extracts obtained from B. phosphorescens by freezing and thawing, and in the alkaline extracts of B. phosphorescens and yeast, resembles the "cytochrome c" of Hill and Keilin (6) and the "porphyratin B" of Schumm (7) in giving absorption bands at mmicro 552-550 and 522-520) but shows in addition a band about 575, as in the(More)
1. The destruction which complement undergoes on being heated in dilution in distilled water is least at a reaction between pH 6.1 and 6.4. This depends upon the relative preservation of the midpiece function at this point. This reaction represents probably the isoelectric point of a compound of the euglobulin with some substance present also in serum. 2.(More)
We conclude therefore that this series of eleven lactose-negative organisms of the Friedländer type, grouped together by Perkins on the basis of fermentation reactions represents a single biological group. It can be distinguished from Bacillus aerogenes and other similar bacilli by cultural, fermentative, and serological reactions. There appears to be a(More)
It has been shown, within the probable limit of error of the methods of measurement employed, that the Donnan equilibrium determines the distribution of H and Cl ions between the cell and the surrounding fluid. This equilibrium is a consequence of the impermeability of the cell membrane to the inorganic cations of the cell. The mechanism responsible for(More)
1. The addition of blood serum displaces the optimum for agglutination of red blood cells in a salt-free medium to the reaction characteristic of flocculation of the serum euglobulin. 2. This effect is not due merely to a mechanical entanglement of the cells by the precipitating euglobulin, since at reactions at which the latter is soluble it protects the(More)
Gillespie (1) was the first to observe that an indifferent metallic electrode placed in a culture of bacteria manifests a negative drift in potential. Potter (2) had found previously that an electrode in an inoculated portion of culture medium is negative to an electrode in an uninoculated portion of the same medium, when the two portions are separated by a(More)
1. The water-insoluble globulin with which hemolysin is associated, may be separated from immune serum or plasma by dilution and simple dialysis at optimal pH. 2. This optimum in plasma is influenced by the presence of the fibrinogen. 3. Fibrinogen carries no immune body, or only an insignificant amount; when present in immune body solutions in other form(More)
1. In a salt-free medium the proportion of the total amount of hemolytic sensitizer present, combined with the homologous cells, reaches a maximum of almost 100 per cent at pH 5.3. On the alkaline side of this point the proportion combined diminishes with the alkalinity and reaches a minimum of approximately 5 per cent at pH 10. On the acid side of pH 5.3(More)