Calvin B. Coulter

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The pigment contained in the extracts obtained from B. phosphorescens by freezing and thawing, and in the alkaline extracts of B. phosphorescens and yeast, resembles the "cytochrome c" of Hill and Keilin (6) and the "porphyratin B" of Schumm (7) in giving absorption bands at mmicro 552-550 and 522-520) but shows in addition a band about 575, as in the(More)
1. The movement of normal and sensitized red blood cells in the electric field is a function of the hydrogen ion concentration. The isoelectric point, at which no movement occurs, corresponds with pH 4.6. 2. On the alkaline side of the isoelectric point the charge carried is negative and increases with the alkalinity. On the acid side the charge is positive(More)
Oxidation-reduction reactions play an important rSle in the metabolism of all living cells. Since such reactions are often brought about in the medium outside of the living cell, a consideration of the oxldation-reduction equilibrium in sterile culture bouillon has seemed desirable because of its possible significance for the reductions manifested by(More)
1. The absorption spectra of a number of proteins in the region 2500 to 3000 A. have been found to comprise from six to nine narrow bands. In consequence of variation in the relative intensity of these bands from protein to protein, the absorption curve has a characteristic configuration for each protein. 2. These bands correspond closely in position with(More)
The optimum for the agglutination of normal sheep cells in isotonic saccharose solution has been given as pH 4.75.1 To correct a possible error in the colorlmetric measurements originally employed electrometric determinations have been made in a similar series of experiments in which graduated amounts of rr/10 to N/40 HC1 have been added to suspensions of(More)
1. In a salt-free medium the proportion of the total amount of hemolytic sensitizer present, combined with the homologous cells, reaches a maximum of almost 100 per cent at pH 5.3. On the alkaline side of this point the proportion combined diminishes with the alkalinity and reaches a minimum of approximately 5 per cent at pH 10. On the acid side of pH 5.3(More)
1. The reduction potentials of B. typhosus in culture in bouillon which is given access to atmospheric oxygen show a negative drift that attains the values found in sterile bouillon when deaerated with nitrogen: Eh -0.085 to -0.095 volt at pH 7.6. The potential reaches this level after 6 to 8 hours incubation, and is maintained at this point for several(More)
It has been shown, within the probable limit of error of the methods of measurement employed, that the Donnan equilibrium determines the distribution of H and Cl ions between the cell and the surrounding fluid. This equilibrium is a consequence of the impermeability of the cell membrane to the inorganic cations of the cell. The mechanism responsible for(More)
We conclude therefore that this series of eleven lactose-negative organisms of the Friedländer type, grouped together by Perkins on the basis of fermentation reactions represents a single biological group. It can be distinguished from Bacillus aerogenes and other similar bacilli by cultural, fermentative, and serological reactions. There appears to be a(More)
1. The addition of blood serum displaces the optimum for agglutination of red blood cells in a salt-free medium to the reaction characteristic of flocculation of the serum euglobulin. 2. This effect is not due merely to a mechanical entanglement of the cells by the precipitating euglobulin, since at reactions at which the latter is soluble it protects the(More)